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17-10078

EZ-Magna ChIP® HT96 Chromatin Immunoprecipitation Kit

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About This Item

NACRES:
NA.84
UNSPSC Code:
41105331
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technique(s)

immunoprecipitation (IP): suitable

Quality Level

100

General description

The EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument. Each kit includes a complete set of validated, quality controlled reagents, positive and negative control antibodies and validated primer set plus a detailed protocol.

Application

The EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format.

Features and Benefits

  • Complete set of materials for up to 96 ChIP reactions
  • Protein A+G bead blend for ChIP with a broader range of antibodies than A or G alone
  • Low Chromatin requirements: 10, 000 to 100, 000 cells per reaction
  • Includes negative and positive control antibodies and control primer set to simplify validation of experimental procedure
  • Optimized streamlined protocol with only a single buffer for sonication, IP, or wash; and protocols for automated liquid handling systemsProtocols for using cells or tissues
  • Direct analysis of DNA without additional clean-up steps
  • Compatible with ChIPAb+ validated antibody and primer sets

Packaging

Kit capacity: 96 chromatin immunoprecipitation assays

Other Notes

Magna ChIP protein A/G magnetic beads;HT96 Nuclei Isolation Buffer ;HT96 ChIP Buffer (Sonication/ChIP/Wash);Low Stringency IP Wash Buffer ;HT96 ChIP Elution Buffer ;Proteinase K Solution;Protease Inhibitor Cocktail III;10X Glycine ;10X PBS ;96 Well ChIP Plate ;96 Well Thermal Plate;Plate Seal;Strip Caps;Anti-Trimethyl-Histone H3 (Lys4) -positive control antibody;Normal Rabbit IgG -negative control antibody;GAPDH Primers - PCR positive control

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Magna ChIP™ HT96 Kits for high throughput chromatin immunoprecipitation.

Magna ChIP™ HT96 Kits for high throughput chromatin immunoprecipitation

Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals

Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA- and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. In a typical ChIP experiment either protein A or G is selected for enrichment depending on the antibody isotype. However, proteins A and G possess differing affinities for human and mouse IgGs. Complicating this choice, for some antibody isotypes there is affinity for both protein A and G. In addition, we have observed that independent of the isotype the affinity of a specific antibody for protein A or G can vary depending on the specific clone, purification method, and source.

96 well Template 2 Up
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