Application
Research Category
Cell Signaling
Used to detect/quantify:GAPDH (Total)
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Analyze total GAPDH protein levels in cell lysate samples to support normalization in multiplex assays and ensure accurate interpretation of protein expression data across experimental conditions.
Analytes Included: GAPDH (total)
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility and other details.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
General description
The MILLIPLEX MAP Total GAPDH Magnetic Bead MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit to detect the presence of total GAPDH in cell lysates using the Luminex® 100 IS, 200, or HTS system. Each MAPmates pair is ordered individually and may be combined for simultaneous multiplex analysis of cellular events. The MILLIPLEX MAP Cell Signaling Buffer and Detection Kit is ordered separately and consists of a common set of reagents needed for performing MAPmates assays. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit contains sufficient reagents for 100 individual assays. The MILLIPLEX MAP HeLa Cell Lysate: Unstimulated included in the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit may be utilized as a unstimulated control for this target.
This panel measures total GAPDH protein levels, a widely used housekeeping protein for normalization in cell signaling studies. GAPDH plays a central role in glycolysis and is commonly used as a loading control in protein expression analysis. The panel supports accurate quantification of GAPDH in cell lysates, enabling normalization across samples in multiplex assays and ensuring reliable interpretation of signaling data
Other Notes
MILLIPLEX Late Aptosis Panel, 48-670MAG
Preparation Note
Recommended storage for kit components is 2 - 8°C
Legal Information
Luminex is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Features and Benefits
- Quantifies total GAPDH protein levels in cell lysates
- Ideal as a housekeeping protein for normalization in signaling studies
- Supports accurate comparison of protein expression across samples
- High sensitivity and reproducibility