Application
Research Category
Cell Signaling
Used to detect/quantify:β-Tubulin
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Analyze total β-Tubulin protein levels in cell lysate samples to support normalization in multiplex assays and enhance the reliability of comparative protein expression studies.
Analytes Included: β-Tubulin (total)
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, and other details.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
General description
The MILLIPLEX MAP Total ß-Tubulin Magnetic Bead MAPmates pair is used in conjunction with the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit to detect the presence of total b-Tubulin in cell lysates using the Luminex® 100 IS, 200, or HTS system. Each MAPmates pair is ordered individually and may be combined for simultaneous multiplex analysis of cellular events. The MILLIPLEX MAP Cell Signaling Buffer and Detection Kit is ordered separately and consists of a common set of reagents needed for performing MAPmates assays. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit contains sufficient reagents for 100 individual assays. The MILLIPLEX MAP HeLa Cell Lysate: Unstimulated included in the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit may be utilized as a unstimulated control for this target.
This panel targets total β-Tubulin, a key structural protein of the cytoskeleton and a standard loading control in protein expression studies. β-Tubulin is essential for maintaining cell shape, intracellular transport, and mitotic spindle formation. The panel enables precise quantification of β-Tubulin in cell lysates, supporting normalization in multiplex assays and enhancing the accuracy of comparative protein analysis.
Legal Information
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
Luminex is a registered trademark of Luminex Corp
xMAP is a registered trademark of Luminex Corp
Preparation Note
Recommended storage for kit components is 2 - 8°C
Features and Benefits
- Measures total β-Tubulin protein levels in cell lysates
- Serves as a reliable loading control for normalization in multiplex assays
- Essential for studies involving cytoskeletal dynamics and protein expression
- High sensitivity and reproducibility