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Merck

48-611MAG

MILLIPLEX® Akt/mTOR Signaling Panel

Premixed Akt/mTOR Phosphoprotein 11-Plex Cell Signaling Panel

Synonym(s):

Akt/mTOR Signaling immunoassay, Luminex® Cell Signaling Assay, Millipore Cell Signaling Assay, Signal Transduction Assay, 11-plex assay, Akt mTOR assay, Akt phosphorylation, PI3K/Akt/mTOR pathway, cell signaling multiplex, kinase signaling panel, mTOR signaling, phospho-Akt, phospho-mTOR

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About This Item

NACRES:
NA.47
UNSPSC Code:
12161503

Key Documents

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Product Name

MILLIPLEX® Akt/mTOR Signaling Panel, Premixed Akt/mTOR Phosphoprotein 11-Plex Cell Signaling Panel

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

packaging

1 ea of

technique(s)

multiplexing: suitable

input

cell lysate

detection method

fluorometric (Luminex® xMAP®technolog)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Features and Benefits

  • Broad coverage of the PI3K/Akt/mTOR signaling pathway
  • Detects multiple phosphorylation sites and total proteins
  • Enables pathway mapping and mechanistic studies
  • High multiplexing reduces sample and reagent consumption
  • Optimized for reproducibility and sensitivity

General description

This panel includes key phosphoproteins from the PI3K/Akt/mTOR pathway such as Akt (pS473, pT308), mTOR (pS2448), p70 S6K, RPS6, 4E-BP1, and GSK-3α/β. These analytes regulate cell survival, growth, protein synthesis, and metabolism through coordinated phosphorylation events.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 11 analytes in cell lysate samples.

Analytes Included: Akt (pS473), Akt (pT308), GSK-3α/β (pS21/S9), mTOR (pS2448), p70 S6K (pT412), p70 S6K (pT389), p70 S6K (Total), RPS6 (pS235/S236), RPS6 (pS240/S244), RPS6 (Total), 4E-BP1 (pT37/T46)

Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).

signalword

Danger

Storage Class

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
48-611MAG04053252757501

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