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Merck

48-684MAG

MILLIPLEX® Ras-Raf Oncoprotein Signaling Panel

Premixed Human Ras-Raf Oncoprotein 6-Plex Cell Signaling Panel

Synonym(s):

Cell Signaling Assay, MAPK cascade assay, MEK1/ERK1/2 panel, Raf kinase assay, Ras pathway ELISA, Ras-Raf assay, Ras-Raf phosphoprotein panel, oncogene signaling multiplex, phospho-Raf

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About This Item

NACRES:
NA.47
UNSPSC Code:
12161503

Key Documents

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Product Name

MILLIPLEX® Ras-Raf Oncoprotein Signaling Panel, Premixed Human Ras-Raf Oncoprotein 6-Plex Cell Signaling Panel

quality

100
200

species reactivity

human

manufacturer/tradename

Milliplex®

packaging

1 ea of

technique(s)

multiplexing: suitable

input

cell lysate

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany

Features and Benefits

  • Simultaneously detect 6 phosphorylated proteins in the Ras-Raf-MEK-ERK signaling cascade
  • Includes c-Raf, MEK1, ERK1/2, and p90RSK
  • Magnetic bead-based format ensures reproducibility and efficient processing
  • Compatible with cell lysates from cancer, proliferation, and signal transduction studies
  • Ideal for oncology, targeted therapy research, and MAPK pathway analysis
  • Fast, convenient alternative to Western Blotting and immunoprecipitation procedures"

General description

This panel targets phosphorylated proteins in the Ras-Raf signaling cascade, a key pathway in cell proliferation and oncogenesis. It includes c-Raf (pS338), MEK1 (pS217/pS221), ERK1/2 (pT185/pY187), and downstream effectors such as p90RSK (pT359/pS363). Dysregulation of this pathway is commonly associated with various cancers, making this panel ideal for oncology and signal transduction research.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 6 analytes in cell lysate samples.

Analytes Included: c-Raf (Phospho-Ser338), MEK1 (Phospho-Ser217/221), ERK1/2 (Phospho-Thr185/Tyr187), p90RSK (Phospho-Thr359/Ser363)

Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).

signalword

Danger

Storage Class

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocol- 48-684MAG

Global Trade Item Number

SKUGTIN
48-684MAG04061841049098

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