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Merck

48-690MAG

MILLIPLEX® T-Cell Receptor Signaling Panel

Premixed T-Cell Receptor Phosphoprotein 7-Plex Cell Signaling Panel

Synonym(s):

7-Plex Magnetic Bead Kit, Magnetic Bead Kit, T-Cell Receptor Signaling Kit, T-cell phosphoprotein assay, T-cell receptor multiplex assay, TCR phosphorylation detection, TCR signaling panel, adaptive immunity signaling biomarkers, rat immune activation panel

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000

Key Documents

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Quality Level

200

species reactivity

human

technique(s)

multiplexing: suitable

input

cell lysate

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

assay range

inter-assay cv: 4-17%
intra-assay cv: 3-8%

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

This panel includes phosphorylated proteins central to T-cell receptor signaling and immune activation. CD3ζ (pY142) initiates TCR signaling upon antigen recognition. LAT (pY191) and SLP-76 (pY128) serve as scaffolding proteins for downstream signaling. Lck (pY394) and ZAP70 (pY319) are tyrosine kinases that propagate TCR signals. PLCγ1 (pY783) mediates calcium signaling, while ERK (pT185/pY187) links TCR activation to gene expression and cell proliferation.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 7 analytes in cell lysate samples.

Analytes Included: CD3ζ (pY142), LAT (pY191), Lck (pY394), PLCγ1 (pY783), ZAP70 (pY319), SLP-76 (pY128), ERK (pT185/pY187)

Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).

Features and Benefits

  • Simultaneously detect 7 phosphorylated proteins involved in T-cell receptor signaling
  • Includes CD3ζ, LAT, Lck, ZAP70, PLCγ1, SLP-76, and ERK for comprehensive immune profiling
  • Magnetic bead-based format ensures reproducibility and efficient processing
  • Compatible with cell lysates from T-cell activation and immunotherapy studies
  • Ideal for adaptive immunity, immune modulation, and cell signaling research
  • Fast, convenient alternative to Western Blotting and immunoprecipitation procedures

Legal Information

Luminex is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

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Danger

Storage Class

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
48-690MAG04053252697210

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