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71187

Insect PopCulture® Reagent

Protein extraction directly from insect cell cultures

Synonyme(s) :

PopCulture reagent

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A propos de cet article

NACRES:
NA.77
UNSPSC Code:
41106511
Service technique
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Quality Segment

100

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

input

plant, fungi and insect sample(s)

shipped in

ambient

storage temp.

10-30°C

General description

Insect POPCULTURE® Reagent is a detergent-based lysis reagent specifically formulated for total insect cell culture extraction without the need for centrifugation. The improved method recovers both protein released into the medium and intracellular protein, increasing processing efficiency and target protein yields (Loomis 2002). It is amenable for automated expression-level screening and is fully compatible with the Ni-NTA His•Bind<TMSYMBOL></TMSYMBOL> affinity purification method. Insect PopCulture reagent can be used for protein extraction from insect cells grown in suspension and adherent cells grown on tissue culture plates.

Features and Benefits

  • No need to separate cells from culture media
  • No need to clarify cell extracts prior to purification
  • Higher protein yield due to target protein recovery from both medium and cells
  • Direct affinity adsorption of target proteins to IMAC affinity resin from the total culture extract
  • Compatible with protease inhibitor cocktails
  • Ideal for high-throughput, expression-level screening and protein purification
  • Compatible with transfected and baculovirus-infected insect cell cultures

Other Notes

Loomis, K., et al. 2002. inNovations15, 16.

Legal Information

This product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
POPCULTURE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Classe de stockage

12 - Non Combustible Liquids

wgk

WGK 3

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