제품 이름
EDANS NovaTag®_resin, Novabiochem®
product line
NovaSyn® TG
Novabiochem®
form
beads
reaction suitability
reaction type: Fmoc solid-phase peptide synthesis
manufacturer/tradename
Novabiochem®
application(s)
peptide synthesis
functional group
amine
storage temp.
15-25°C
Quality Level
Analysis Note
Color (visual): white to yellow to brown
Appearance of substance (visual): beads
Loading (determined from the substitution of the Fmoc-Leu loaded resin): 0.30 - 0.60 mmol/g
Swelling Volume (in DMF): lot specific result
The polymer matrix is copoly styrene - 1 & DVB 100 - 200 mesh
Appearance of substance (visual): beads
Loading (determined from the substitution of the Fmoc-Leu loaded resin): 0.30 - 0.60 mmol/g
Swelling Volume (in DMF): lot specific result
The polymer matrix is copoly styrene - 1 & DVB 100 - 200 mesh
General description
The EDANS/dabcyl fluorophore-quencher pair is one of the most commonly-used for FRET peptides, owing to excellent spectral overlap between the emission spectrum of EDANS and absorbance spectrum of dabcyl [1]. The introduction of the EDANS moiety is traditionally achieved either by coupling of a peptide fragment to EDANS in solution [1, 2] or through the use of a pre-formed derivative, such as Fmoc-Glu(EDANS)-OH [3]. Novabiochem′s EDANS resin enables for the first time the direct synthesis of C-terminally EDANS-labeled peptides by solid phase synthesis [4]. Loading of the first residue requires acylation of the resin-bound secondary amine and is, therefore, best carried out using HATU activation. Chain extension and cleavage can then be effected using standard methods. EDANS NovaTag resin has been recently employed to prepare fluorescently labeled aminoalkane diphenyl phosphonate affinity probes for chymotrypsin- and elastase-like serine proteases [5].
Associated Protocols and Technical Articles
Protocols for Loading of Peptide Synthesis Resins Literature references
[1] G. T. Wang, et al. (1990) Tetrahedron Lett., 31, 6493.
[2] C. Garcia-Echeverria & D. H. Rich (1992) Febs Lett., 297, 100.
[3] J. W. Drijfhout, et al. in ′Peptides, Chemistry, Structure& Biology, Proc. 14th American Peptide Symposium′, P. T. P. Kaumaya &R. S. Hodges (Eds), Kingswinford, Mayflower Scientific Ltd., 1996, pp. 129.
[4] J. Beythien & P. White (2005) Tetrahedron Lett., 46,101.
[5] B. F. Gilmore, et al. (2009) Bioconjugate Chem., 20, 2098.
Associated Protocols and Technical Articles
Protocols for Loading of Peptide Synthesis Resins Literature references
[1] G. T. Wang, et al. (1990) Tetrahedron Lett., 31, 6493.
[2] C. Garcia-Echeverria & D. H. Rich (1992) Febs Lett., 297, 100.
[3] J. W. Drijfhout, et al. in ′Peptides, Chemistry, Structure& Biology, Proc. 14th American Peptide Symposium′, P. T. P. Kaumaya &R. S. Hodges (Eds), Kingswinford, Mayflower Scientific Ltd., 1996, pp. 129.
[4] J. Beythien & P. White (2005) Tetrahedron Lett., 46,101.
[5] B. F. Gilmore, et al. (2009) Bioconjugate Chem., 20, 2098.
Other Notes
Replaces: 04-12-3904
Legal Information
NOVASYN is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVATAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 8.55054.8500 | 04027269111625 |
| 8.55054.8100 | 04027269111618 |
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