GF156
B18R, Human Recombinant Carrier-Free
liquid, >98% (SDS-PAGE), <0.05 ng/μg cytokine (LAL test)
Synonym(s):
B18R protein, Recombinant B18R
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.75
eCl@ss:
32160405
Product Name
B18R, Human Recombinant Carrier-Free, liquid, >98% (SDS-PAGE), <0.05 ng/μg cytokine (LAL test)
biological source
human
assay
>98% (SDS-PAGE)
form
liquid
specific activity
≥1.3 x 10e6 units/mg protein
mol wt
Mw 38,389 Da
concentration
0.5 mg/mL
technique(s)
cell culture | stem cell: suitable
impurities
<0.05 ng/μg cytokine (LAL test)
input
sample type induced pluripotent stem cell(s)
solubility
water: soluble
suitability
suitable for molecular biology
shipped in
dry ice
Quality Level
Analysis Note
The DTT-reduced protein migrates as a 48 kDa polypeptide on SDS-PAGE due to glycosylation. The non-reduced protein migrates as a 46 kDa polypeptide on SDS-PAGE due to internal cystines.
Application
B18R, Human RecombinantCarrier-Free has been used as a decoy receptor to study the intrinsic antiviralactivity of recombinant soluble IFNα/β cell surface receptor 2 (sIFNAR2). Ithas also been used in the generation and maintenance of induced pluripotentstem cells (iPSCs) and human IPSCs.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Product Source: Insect cells infected with baculovirus: amino acids His20-Glu 351 with a His-tag attached of Vaccinia Virus B18R.
Research area: Immuno and CKS B18R is a virally-encoded protein that acts as a decoy receptor for Type 1 Interferons. B18R enables increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs via direct delivery of synthetic mRNA′s. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta.
Physical form
Sterile liquid;10 mM phosphate, 0.5 M NaCl, pH 7.5 buffer and is filtered with a 0.2µm filter before vialing.
Preparation Note
For best recovery, quick-spin vial prior to opening. Thaw on ice. While on ice, aliquot B18R protein into sterile, nuclease-free, low protein-binding eppendorf tubes and store at -80°C. Limit repeated freeze and thaw cycle. Use in a sterile environment.
Further dilutions should be made into appropriate buffer or medium.
Further dilutions should be made into appropriate buffer or medium.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Irene L Llorente et al.
Stem cell research, 55, 102458-102458 (2021-07-19)
White matter stroke (WMS) occurs as small infarcts in deep penetrating blood vessels in the brain and affects the regions of the brain that carry connections, termed the subcortical white matter. WMS progresses over years and has devastating clinical consequences.
Isaac Hurtado-Guerrero et al.
Journal of clinical medicine, 9(4) (2020-04-05)
Soluble receptors of cytokines are able to modify cytokine activities and therefore the immune system, and some have intrinsic biological activities without mediation from their cytokines. The soluble interferon beta (IFN-ß) receptor is generated through alternative splicing of IFNAR2 and
Roni A Hazim et al.
Stem cell research & therapy, 8(1), 217-217 (2017-10-04)
Dysfunction of the retinal pigment epithelium (RPE) is implicated in numerous forms of retinal degeneration. The readily accessible environment of the eye makes it particularly suitable for the transplantation of RPE cells, which can now be derived from autologous induced
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| GF156 | 04055977116397 |
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