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Merck

MAB1273B

Anti-Mitochondria Antibody, clone 113-1, Biotin Conjugate

clone 113-1, from mouse, biotin conjugate

Synonyme(s) :

Human Mitochondria

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A propos de cet article

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
biotin conjugate
Clone:
113-1, monoclonal
Application:
ICC, IHC
Citations:
2
Service technique
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biological source

mouse

Quality Segment

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

113-1, monoclonal

species reactivity

human (mitochondria protein)

should not react with

rat (mitochondria protein), mouse (mitochondria protein)

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

General description

60 kDa calculated
This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions. MAB1273B recognizes a 65 kD protein by immunoprecipitation and produces the stringy, "spaghetti-like", staining patten in the cytoplasm of human cells.

Immunogen

Surface of intact human mitochondria

Application

Detect Mitochondrial Marker using this mouse monoclonal antibody, Anti-Mitochondria Antibody, clone 113-1, Biotin Conjugate validated for use in ICC & IHC.
Evaluated by Immunocytochemistry in primary mouse embryonic fibroblasts (PMEFs).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody did not detect mitrochondria in primary mouse embryonic fibroblasts (PMEFs). Evaluated by Immunohistochemistry in human kidney tissue.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected mitrochondria in human kidney tissue.
Research Category
Stem Cell Research

Cell Structure
Research Sub Category
Developmental Neuroscience

Organelle & Cell Markers

Biochem/physiol Actions

This antibody recognizes human. Antibody is not reactive with mouse or rodent.

Physical form

Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.

Preparation Note

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Human adipose mesenchymal stem cells (SCC038).
Evaluated by Immunocytochemistry in human adipose mesenchymal stem cells (SCC038).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in human adipose mesenchymal stem cells (SCC038).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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