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Merck

MRCF0R030

Microcon® Centrifugal Filters

NMWCO 30 kDa, Ultracel® regenerated cellulose membrane (low binding), sample volume 0.5 mL

Synonym(s):

Centrifuge concentrator

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About This Item

UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101

Product Name

Microcon® Centrifugal Filters, NMWCO 30 kDa, Ultracel® regenerated cellulose membrane (low binding), sample volume 0.5 mL

material

Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)

sterility

non-sterile

feature

holdup volume> 10 μL

packaging

pkg of 100 ea

manufacturer/tradename

Microcon®

parameter

0.5 mL sample volume

technique(s)

DNA purification: suitable
RNA purification: suitable
protein extraction: suitable
protein purification: suitable

L

4.50 cm (1.8 in.)

diam.

12.3 mm

filtration area

0.32 cm2

working volume

0.5 mL

pore size

30 kDa NMWCO

Quality Level

400

shipped in

ambient

Related Categories

General description

Simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. With the low-binding Ultracel membrane, Microcon filters offer:
•Typical recoveries of greater than 95%, even for dilute solutions
•Reverse spin to maximize recovery, even in the smallest samples
•Convenient storage of filtrate or concentrated sample in standard microfuge tube
•Concentration factors up to 100X

Features:
•High recovery for small volumes with reverse spin (concentration factor less than 20X)
•Low-binding Ultracel membrane
•Fast processing

Sample applications:
•Recovery of genomic DNA for forensic applications
•Concentration and desalting of nucleic acids (high recovery alternative to ethanol precipitation)
•Removal of primers from amplified DNA
•Easy filtrate recovery, exploiting the flat membrane design

Other Notes

Replaces: 42410

Legal Information

MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10-13 - German Storage Class 10 to 13

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sarah K Stumper et al.
eLife, 8 (2019-02-06)
RNA polymerases (RNAPs) contain a conserved 'secondary channel' which binds regulatory factors that modulate transcription initiation. In Escherichia coli, the secondary channel factors (SCFs) GreB and DksA both repress ribosomal RNA (rRNA) transcription, but SCF loading and repression mechanisms are
Flavio Oliveira et al.
Toxins, 12(3) (2020-04-05)
Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species
Michael D Ward et al.
Clinical proteomics, 17, 11-11 (2020-03-21)
Detection of viral ribo-nucleic acid (RNA) via real-time polymerase chain reaction (RT-PCR) is the gold standard for the detection of Ebola virus (EBOV) during acute infection. However, the earliest window for viral RNA detection in blood samples is 48-72 h post-onset
Sergey Radko et al.
Biomedicines, 8(5) (2020-05-28)
Selected reaction monitoring (SRM) is a mass spectrometric technique characterized by the exceptionally high selectivity and sensitivity of protein detection. However, even with this technique, the quantitative detection of low- and ultralow-abundance proteins in blood plasma, which is of great
Chung-Hsuan Kao et al.
Molecular and cellular biology, 40(12) (2020-03-25)
Maintenance of protein homeostasis in eukaryotes under normal growth and stress conditions requires the functions of Hsp70 chaperones and associated cochaperones. Here, we investigate an evolutionarily conserved serine phosphorylation that occurs at the site of communication between the nucleotide-binding and
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The new dual cycle EtO-treated Microcon® DNA Fast Flow PCR Grade devices enable centrifugal concentration at a similar rate as the Microcon® DNA Fast Flow devices, but the EtO treatment greatly reduces the risk that no uncontrolled contaminating nucleic acids enter the analysis process. Both Microcon® devices exhibit faster flow rate (volume filtered per minute) compared to competitor centrifugal products.

Trust Microcon®filters for DNA and protein samples.

Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of any macromolecule, using any centrifuge that can accept 1.5 mL tubes. The low nonspecific binding characteristics of the Ultracel® membrane and the other device components, coupled with its medical-grade o-ring seal, allows the device to accommodate several wash steps with minimal sample loss.

User Guide-Microcon® Centrifugal Filter Devices

Global Trade Item Number

SKUGTIN
MRCF0R03004053252579707

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