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SCM110

Human ES/iPS Neural Induction Medium

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UNSPSC Code:
41106200
NACRES:
NA.32
eCl@ss:
32160801
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form

liquid

Quality Segment

100

technique(s)

cell differentiation: suitable

General description

EMD Millipore′s Human ES/iPS Neural Induction Mediium is an serum free/cheimcally defined optimized media for the rapid production of expandable neural progenitor cells from pluripotent human ES and iPS cells. The media relies on both small molecules neural inducers and supplements to generate a highly enriched population of expandable neural progenitors based on establish protocols. Neural progenitors are generated 10 days from starting cultures of traditional feeder-based and/or feeder-free cultures of undifferentiated human ES/iPS cells and can be expanded for over 3-5 passages resulting in a minimum 20-fold expansion. Typically, from 1 well of a 6-well plate containing an estimated 500,000 pluripotent human ES/iPS, approximately 5-10 million neural progenitors can be obtained after 3 passages. The resulting neural progenitor cells are multipotent and can be further differentiated to specific neural cell subtypes of interest.

Preparation Note

Store at -20C. Good for 3 months from date of receipt or until expiration date on bottle when reagents are handled and stored appropriately. Please note: The supplemented ES/iPS Neural Induction Medium should be stored at 2 to 8C for up to 4 weeks.

Other Notes

1) Neural Induction Basal Medium (Part No. CS210991). One 125 mL bottle. 2) Neural Supplement 1 (50X) (Part No. CS210992). One 2.5 mL bottle. 3) Neuro2 Medium Supplement (Part No. SCM012-S). One 1.25 mL bottle. 4) GSK3 and TGFbR Inhibitor Cocktail (1000X) (Part No. CS210983-150UL). One 150 µL vial. 5) AMPK Inhibitor (1000X) (Part No. CS210995-60UL). One 60 µL vial.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Classe de stockage

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 2

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Contenu apparenté

User Guide - SCM110
Neural Induction Medium Efficiently Generates Multipotent Neural Progenitors from Human iPS Cells

Dual SMAD inhibition is a well-established method to derive neural progenitor cells from both human ES and iPS cells. This protocol uses two SMAD inhibitors, Noggin and SB431542, to drive the rapid differentiation of ES/iPS cells into a highly enriched population of NPCs. Noggin acts as a BMP inhibitor and SB431542 inhibits the Lefty/Activin/TGFβ pathways by blocking the phosphorylation of ALK4, ALK5, and ALK7 receptors. In an effort to make a more defined and optimized neuronal differentiation protocol, Li and colleagues modified the original protocol to establish a completely small molecule-based differentiation method, which relies on three small molecules to inhibit GSK-3β (CHIR99021), TGFβ (SB431542), and Notch (compound E) signaling pathways, along with human LIF3. This new small molecule-based neural differentiation protocol increased neural differentiation kinetics and allowed the derivation of truly multipotent neural stem cells that respond to regional patterning cues specifying forebrain, midbrain, and hindbrain neural and glial subtypes.

Human ES/iPS Neurogenesis Kit
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Numéro d'article de commerce international

RéférenceGTIN
SCM11004053252855108