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Merck

UFC9010

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

greener alternative

sample volume 15 mL, regenerated cellulose membrane, MWCO 10 kDa

別名:

10 kDa MWCO, 15 mL sample volume, Centrifugal concentrator

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この商品について

UNSPSC Code:
41104916
NACRES:
NB.24
eCl@ss:
36100101
Material:
polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing
Pore size:
10 kDa MWCO
Working volume:
≤15 mL , 15 mL
Sterility:
non-sterile
Packaging:
pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg

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Quality Level

400

material

polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing

sterility

non-sterile

product line

Amicon®

packaging

pkg of 24 pkg, pkg of 8 pkg, pkg of 96 pkg

manufacturer/tradename

Amicon® Ultra

sustainability

Greener Alternative Product

parameter

15 mL sample volume

technique(s)

protein extraction: suitable, protein purification: suitable

L

12.1 cm (4.807 in.)

diam.

2.97 cm

filtration area

7.6 cm2

volume

15 mL

working volume

≤15 mL , 15 mL

pore size

10 kDa MWCO

greener alternative category

Re-engineered

shipped in

ambient

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General description

Amicon® Ultra 15 mL centrifugal devices with Ultracel® 10,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

Application

  • Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.

Features and Benefits

  • Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%.
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10-15 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipettor sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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資料

ナノ粒子の限外ろ過

ナノ粒子の精製と製造における、ろ過法とメンブレンフィルターの選定。

タンパク質サンプルの限外ろ過

溶媒交換やサンプル濃縮などのタンパク質サンプル調製のための限外ろ過膜の原理と利点をご覧ください。

Protein Sample Ultrafiltration

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

プロトコル

Extracellular Vesicle Protocol

Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.

グローバルトレードアイテム番号

カタログ番号GTIN
UFC90100804054839076800
UFC90109604054839076640
UFC90102404054839076633

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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