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110116 β1-2,3,4,6-N-Acetylglucosaminidase, Streptococcus pneumoniae, Recombinant, E. coli

110116
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      Description
      OverviewRecombinant, Streptococcus pneumoniae β1-2,3,4,6-N-Acetylglucosaminidase expressed in E. coli. Catalyzes the hydrolysis of β1-2,3,4,6-linked N-acetylglucosamine from complex carbohydrates. Not known to cleave substituted or branched hexosamines. Does not cleave N-acetylgalactosamine residues.
      Catalogue Number110116
      Brand Family Calbiochem®
      SynonymsN-Acetyl-β-D-glycosaminide-N-acetylglucosaminohydrolase
      References
      ReferencesPrime, S., et al. 1996. J. Chromatogr. A. 720, 263.
      Clarke, V.A., et al. 1995. J. Biol. Chem. 270, 8805.
      Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
      Product Information
      CAS number9012-33-3
      Activity≥ 35 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol <i>p</i>-nitrophenol from p-nitrophenyl-β-D-N-acetylglucosaminide per minute at 37°C, pH 5.0.
      EC number3.2.1.30
      FormLiquid
      FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥ 80 units/mg protein
      Physicochemical Information
      ContaminantsEndo-α-N-acetylgalactosaminidase, α- and β-galactosidase, neuraminidases, proteases: none detected.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      β1-2,3,4,6-N-Acetylglucosaminidase, Streptococcus pneumoniae, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      110116

      References

      Reference overview
      Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
      Clarke, V.A., et al. 1995. J. Biol. Chem. 270, 8805.
      Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-April-2008 RFH
      SynonymsN-Acetyl-β-D-glycosaminide-N-acetylglucosaminohydrolase
      DescriptionRecombinant, Streptococcus pneumoniae β1-2,3,4,6-N-Acetylglucosaminidase expressed in E. coli. Catalyzes the hydrolysis of β1-2,3,4,6-linked N-acetylglucosamine from complex carbohydrates. Not known to cleave substituted or branched hexosamines. Does not cleave N-acetylgalactosamine residues.
      FormLiquid
      FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
      Recommended reaction conditions50 mM sodium phosphate buffer, pH 5.0.
      CAS number9012-33-3
      EC number3.2.1.30
      ContaminantsEndo-α-N-acetylgalactosaminidase, α- and β-galactosidase, neuraminidases, proteases: none detected.
      Specific activity≥ 80 units/mg protein
      Activity≥ 35 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-N-acetylglucosaminide per minute at 37°C, pH 5.0.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesPrime, S., et al. 1996. J. Chromatogr. A. 720, 263.
      Clarke, V.A., et al. 1995. J. Biol. Chem. 270, 8805.
      Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.