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MABS2498-100UG Anti-Caspase-8 Antibody, clone C15

Anti-Caspase-8, clone C15, Cat. No. MABS2498, is a mouse monoclonal antibody that detects Caspase-8 and is tested for use in Western Blotting and Immunoprecipitation.

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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2498-100UG
      DescriptionAnti-Caspase-8 Antibody, clone C15
      Alternate Names
      • EC:3.4.22.61
      • CASP-8
      • Apoptotic cysteine protease
      • Apoptotic protease Mch-5
      • CAP4
      • FADD-homologous ICE/ced-3-like protease
      • FADD-like ICE (FLICE)
      Background InformationCaspase-8 (UniProt: Q14790; also known as CASP-8, EC:3.4.22.61, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE (FLICE)) is encoded by the CASP8 (also known as MCH5) gene (Gene ID: 841) in human. Caspase-8 is a member of a family of cysteine-requiring aspartate proteases that participate in the intracellular signaling cascade leading to apoptosis, or programmed cell death. Caspase-8 is recruited to death-inducing signaling complex (DISC), CD95 (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) and is therefore the most upstream caspase in the CD95 apoptotic pathway. Caspase-8 is ubiquitously expressed in various tissues, with highest expression in peripheral blood leukocytes, spleen, thymus and liver. Its barely detectable in brain, testis and skeletal muscle. It is mainly localized in the cytoplasm and upon activation, it can translocate to the membrane-associated DISC, where it undergoes proteolytic activation. It is synthesized as an inactive proenzyme composed of an N-terminal prodomain (aa 1-216) containing two death effector domains (DEDs), followed by a large (p18) (aa 217-374) and a small (p10) (aa 385-479) catalytic subunit. Caspase-8 that binds to the death effector domain (DED) of FADD through an analogous DED domain present in the proform of caspase-8. A total of 9 isoforms are known to be produced by alternative splicing. Studies show that only two of the FLICE isoforms (caspase-8/a and caspase-8/b) were predominantly expressed in cells of different origin. Both isoforms were recruited to the CD95 death-inducing signaling complex and were activated upon CD95 stimulation with similar kinetics. (Ref.: Scaffidi, C., et al. (1997). J Biol Chem. 272(43):26953-8; Tang, D., et al. (2000). J Biol Chem. 275(13):9303-7; Teitz, T., et al. (2000). Nat Med. 6(5):529-35; Chang, D.W., et al. (2002). EMBO J. 21(14):3704-14; Lavrik, I., et al. (2003). Cell Death Differ. 10(1):144-5; Schmitz, I., et al. (2004). J Immunol. 172(4):2194-200; Lavrik, I.N., et al. (2008). J Biol Chem. 283(39):26401-8; Kallenberger, S.M., et al. (2014). Sci Signal. 7(316):ra23; de Reuver, R. et al. (2022). Nature. 607(7920):784-789).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Caspase-8, clone C15, Cat. No. MABS2498, is a mouse monoclonal antibody that detects Caspase-8 and is tested for use in Western Blotting and Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesTested Applications

      Western Blotting Analysis: A 1:1000 dilution of this antibody detected caspase-8 in Hep-G2, Jurkat, and Raji cell lysates.

      Western Blotting Analysis: A representative lot detected Caspase-8 in Western Blotting applications (Scaffidi, C., et al. (1997). J Biol Chem. 272(43):26953-8; Tang, D., et al. (2000). J Biol Chem. 275(13):9303-7; Teitz, T., et al. (2000). Nat Med. 6(5):529-35; Chang, D.W., et al. (2002). EMBO J. 21(14):3704-14; Lavrik, I., et al. (2003). Cell Death Differ. 10(1):144-5; Schmitz, I., et al. (2004). J Immunol. 172(4):2194-200; Lavrik, I.N., et al. (2008). J Biol Chem. 283(39):26401-8; Kallenberger, S.M., et al. (2014). Sci Signal. 7(316):ra23; de Reuver, R. et al. (2022). Nature. 607(7920):784-789).

      Immunoprecipitation Analysis: A representative lot detected Caspase-8 in Immunoprecipitation (Scaffidi, C., et al. (1997). J Biol Chem. 272(43):26953-8; Tang, D., et al. (2000). J Biol Chem. 275(13):9303-7; Teitz, T., et al. (2000). Nat Med. 6(5):529-35; Chang, D.W., et al. (2002). EMBO J. 21(14):3704-14; Lavrik, I., et al. (2003). Cell Death Differ. 10(1):144-5; Schmitz, I., et al. (2004). J Immunol. 172(4):2194-200; Lavrik, I.N., et al. (2008). J Biol Chem. 283(39):26401-8; Kallenberger, S.M., et al. (2014). Sci Signal. 7(316):ra23; de Reuver, R. et al. (2022). Nature. 607(7920):784-789).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-tagged, C-terminal recombinant fragment of human Caspase-8.
      EpitopeC-terminal
      CloneC15
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone C15 is a mouse monoclonal antibody that specifically detects Caspase-8. It targets an epitope within 297 amino acids from the C-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CASP8
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~55 kDa observed; 55.39 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate .

      Western Blotting Analysis: A 1:1000 dilution of this antibody detected caspase-8 in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2498-100UG 04065272161497