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PC680 Anti-Caspase-9, Cleaved (Ab-2) Rabbit pAb

Anti-Caspase-9, Cleaved (Ab-2) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Active Caspase-9

PC680
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~37 kDa processed form of caspase-9 in actinomycin D induced A431 cells.
      Catalogue NumberPC680
      Brand Family Calbiochem®
      SynonymsAnti-Active Caspase-9
      Application Data
      Detection of human cleaved caspase-9 by immunoblotting. Samples: Whole cell lysate from Jurkat cells left untreated (lane 1) and treated with etoposide (lane 2). Primary antibody: Anti-Caspase-9, Cleaved (Ab-2) Rabbit pAb (Cat. No. PC680) (0.5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, 1% BSA, pH 7.2.
      Positive controlJurkat cells treated with etoposide
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1 µg/ml)
      Immunocytochemistry (10-20 µg/ml)
      Application CommentsDoes not recognize full-length caspase-9. Useful for the identification of apoptotic cells in cell populations. To activate caspase-9 treat with an inducer of apoptosis such as actinomycin D at 0.5 µg/ml for 24 h. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids surrounding the Asp³³⁰ cleavage site of human caspase-9
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC680 0

      Documentation

      Anti-Caspase-9, Cleaved (Ab-2) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Caspase-9, Cleaved (Ab-2) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC680
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-October-2007 RFH
      SynonymsAnti-Active Caspase-9
      ApplicationImmunoblotting (1 µg/ml)
      Immunocytochemistry (10-20 µg/ml)
      Application Data
      Detection of human cleaved caspase-9 by immunoblotting. Samples: Whole cell lysate from Jurkat cells left untreated (lane 1) and treated with etoposide (lane 2). Primary antibody: Anti-Caspase-9, Cleaved (Ab-2) Rabbit pAb (Cat. No. PC680) (0.5 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~37 kDa cleaved form of caspase-9.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids surrounding the Asp³³⁰ cleavage site of human caspase-9
      IsotypeIgG
      Specieshuman
      Positive controlJurkat cells treated with etoposide
      FormLiquid
      FormulationIn PBS, 50% glycerol, 1% BSA, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not recognize full-length caspase-9. Useful for the identification of apoptotic cells in cell populations. To activate caspase-9 treat with an inducer of apoptosis such as actinomycin D at 0.5 µg/ml for 24 h. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling