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PH02 Anti-PTP1B (Ab-2) Mouse mAb (AE4-2J)

Anti-PTP1B (Ab-2) Mouse mAb (AE4-2J) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Protein Tyrosine Phosphatase 1B

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~50 kDa PTPase 1B protein.
      Catalogue NumberPH02
      Brand Family Calbiochem®
      SynonymsAnti-Protein Tyrosine Phosphatase 1B
      References
      ReferencesFischer, E.H., et al. 1991. Science 253, 401.
      Pallen, C.J., et al. 1991. Biochem. J. 276, 315.
      Brown-Shimer, S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 5148.
      Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
      Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
      Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
      Positive controlHT-1080 or SW480 cells
      PreservativeNone
      Applications
      Application ReferencesCross Reactivity Brown-Shimer, S., et al. 1992. Cancer Res. 52, 478.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Application CommentsRecognizes only full-length PTPase 1B. May also detect a 90-95 kDa protein by immunoblotting (but not immunoprecipitation) that may be mor intense than PTPase 1B (see application references). Antibody should be titrated for optimal results in individual sample types.
      Biological Information
      Immunogenrecombinant, human protein tyrosine phosphatase 1B (PTPase 1B)
      ImmunogenHuman
      Epitopewithin the C-terminal 120 amino acids
      CloneAE4-2J
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PH02 0

      Documentation

      Anti-PTP1B (Ab-2) Mouse mAb (AE4-2J) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PTP1B (Ab-2) Mouse mAb (AE4-2J) Certificates of Analysis

      TitleLot Number
      PH02

      References

      Reference overview
      Fischer, E.H., et al. 1991. Science 253, 401.
      Pallen, C.J., et al. 1991. Biochem. J. 276, 315.
      Brown-Shimer, S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 5148.
      Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
      Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
      Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.

      Brochure

      Title
      Protein Phosphatases Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-October-2007 RFH
      SynonymsAnti-Protein Tyrosine Phosphatase 1B
      ApplicationImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      DescriptionMouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP2/0 myeloma cells. Recognizes the ~50 kDa full-length PTPase 1B protein.
      BackgroundProtein tyrosine phosphatases (PTPases) make up an expanding family of transmembrane and intracellular proteins, which function to remove phosphate groups from protein phosphoryl-tyrosine residues. PTPase 1B was initially identified as an active intracellular phosphatase from human placenta made up of 321 amino acids. It was subsequently shown to derive from a larger precursor protein containing 435 amino acids. Further examination failed to show alternative splicing or more than a single copy of PTPase 1B in the genome. Purification of a 50 kDa protein with phosphatase activity from human placenta demonstrated the protein to have an amino acid composition similar to that for PTPase 1B and N-terminal analysis revealed an identical sequence. PTPases are involved in regulating processes where protein kinases and protein tyrosine phosphorylation are important. Recently it has been demonstrated that expression of PTPase 1B has profound effects on the transformation induced by the human neu oncogene. Based on this and other observations it has been hypothesized that PTPases have the potential to function as tumor suppressor genes.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human protein tyrosine phosphatase 1B (PTPase 1B)
      Epitopewithin the C-terminal 120 amino acids
      CloneAE4-2J
      IsotypeIgG₁
      Specieshuman, not mouse
      Positive controlHT-1080 or SW480 cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsRecognizes only full-length PTPase 1B. May also detect a 90-95 kDa protein by immunoblotting (but not immunoprecipitation) that may be mor intense than PTPase 1B (see application references). Antibody should be titrated for optimal results in individual sample types.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesFischer, E.H., et al. 1991. Science 253, 401.
      Pallen, C.J., et al. 1991. Biochem. J. 276, 315.
      Brown-Shimer, S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 5148.
      Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
      Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
      Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.
      Application referencesCross Reactivity Brown-Shimer, S., et al. 1992. Cancer Res. 52, 478.