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PC491 Anti-TACE (807 - 823) Rabbit pAb

This Anti-TACE (807 - 823) Rabbit pAb is validated for use in Immunoblotting for the detection of TACE (807 - 823).

Anti-TACE (807 - 823) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TNF-α Converting Enzyme, Anti-ADAM17

PC491
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~130 kDa TACE protein in HeLa and Jurkat cells.
      Catalogue NumberPC491
      Brand Family Calbiochem®
      SynonymsAnti-TNF-α Converting Enzyme, Anti-ADAM17
      Application Data
      Detection of human TACE by immunoblotting. Samples: Whole cell lysate from HeLa cells (lanes 1,3) or Jurkat cells (lanes 2,4). Primary antibody: Anti-TACE (807 - 823) Rabbit pAb (Cat. No. PC491) pre-incubated without blocking peptide (lanes 1,2) or with blocking peptide (lanes 3,4) (1:500). Detection: chemiluminescence.
      References
      ReferencesMizul, Y., et al. 1999. Gene. 233, 67.
      Buxbaum, J.D., et al. 1998. J. Biol. Chem. 273, 27765.
      Giegel, D.A. and Cheplinm, D.D. 1998. Agents Actions Suppl. 491, 1.
      Peschon, J.J., et al. 1998. Science 282, 1281.
      Black, R.A., et al. 1997. Nature 385, 729.
      Moss, M.L., et al. 1997. Nature 385, 733.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHeLa or Jurkat cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:500-1:2000)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (ASFKLQRQNRVDSKETE) corresponding to amino acids 807-823 of human TACE
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC491 0

      Documentation

      Anti-TACE (807 - 823) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-TACE (807 - 823) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC491

      References

      Reference overview
      Mizul, Y., et al. 1999. Gene. 233, 67.
      Buxbaum, J.D., et al. 1998. J. Biol. Chem. 273, 27765.
      Giegel, D.A. and Cheplinm, D.D. 1998. Agents Actions Suppl. 491, 1.
      Peschon, J.J., et al. 1998. Science 282, 1281.
      Black, R.A., et al. 1997. Nature 385, 729.
      Moss, M.L., et al. 1997. Nature 385, 733.

      Citations

      Title
    • Daniel W. Lambert, et al. (2005) Tumor Necrosis Factor- Convertase (ADAM17) Mediates Regulated Ectodomain Shedding of the Severe-acute Respiratory Syndrome-Coronavirus (SARS-CoV) Receptor, Angiotensin-converting Enzyme-2 (ACE2). Journal of Biological Chemistry 280, 30113-30119.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-October-2007 RFH
      SynonymsAnti-TNF-α Converting Enzyme, Anti-ADAM17
      ApplicationImmunoblotting (1:500-1:2000)
      Application Data
      Detection of human TACE by immunoblotting. Samples: Whole cell lysate from HeLa cells (lanes 1,3) or Jurkat cells (lanes 2,4). Primary antibody: Anti-TACE (807 - 823) Rabbit pAb (Cat. No. PC491) pre-incubated without blocking peptide (lanes 1,2) or with blocking peptide (lanes 3,4) (1:500). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody purified by ion exchange chromatography. Recognizes the TACE protein.
      BackgroundTumor-necrosis factor-α is a proinflammatory cytokine that contributes to a variety of inflammatory disease responses and programmed cell death. TNF-α is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-α. TNF-α converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE, a ~130 kDa protein, is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFα, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (ASFKLQRQNRVDSKETE) corresponding to amino acids 807-823 of human TACE
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlHeLa or Jurkat cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMizul, Y., et al. 1999. Gene. 233, 67.
      Buxbaum, J.D., et al. 1998. J. Biol. Chem. 273, 27765.
      Giegel, D.A. and Cheplinm, D.D. 1998. Agents Actions Suppl. 491, 1.
      Peschon, J.J., et al. 1998. Science 282, 1281.
      Black, R.A., et al. 1997. Nature 385, 729.
      Moss, M.L., et al. 1997. Nature 385, 733.
      Citation
    • Daniel W. Lambert, et al. (2005) Tumor Necrosis Factor- Convertase (ADAM17) Mediates Regulated Ectodomain Shedding of the Severe-acute Respiratory Syndrome-Coronavirus (SARS-CoV) Receptor, Angiotensin-converting Enzyme-2 (ACE2). Journal of Biological Chemistry 280, 30113-30119.
      		•