Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Immobilon® Membranes, Sandwiches and Blotting Filter Paper
Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications.More
Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications. Less
Mobile Phase Preparation for UHPLC: Membrane Filtration Method Affects System Performance and Leaching of Extractable Impurities Subodh Kulkarn(1), Jesmi George(2) and Vivek Joshi(2) (1) Millipore India Pvt. Ltd., Bioscience Division, 50A, 2nd Phase, Ring Road, Peenya, Bangalore, India 560058 (2) Millipore Corp., Bioscience Division, 17 Cherry Hill Drive, Danvers, MA 01923 LCGC
2010
UHPLC/UPLC® is a revolutionary chromatography technique that is gaining wide acceptance among researchers due to improved resolution, shorter chromatographic runs, and the capability for doing fast method development. The presence of sub-2 µm particles in UHPLC columns provide these benefits but also poses challenges in sample and mobile phase preparation. Particulate impurities in the sample or mobile phase can cause backpressure buildup in the UHPLC system, causing system failure. In fact, most UHPLC instrument vendors recommend filtration of mobile phase using 0.2 µm filters, but there is a lack of data showing the benefits of filtration. In this article we describe the filtration of mobile phases through syringe filters of varying pore size and membrane type, followed by analysis by UHPLC and mass spectrometry. Our results clearly indicate that filtration of mobile phase components using the optimal membrane filter will help protect UHPLC systems from particulate impurities that may clog and shut down the system, increase the sensitivity of detection, and improve the accuracy of quantitation.
Quantitation of Protein on gels and blots by infrared fluorescence of Coomassie blue and fast green Luo S., Wehr N.B., Levine R.L. Analytical Biochemistry:350 (2006):233-238
2006
Immunoblotting (Western)
Role of the Small Heat Shock Proteins in Regulating Vascular Smooth Muscle Tone McLemore E.C., Tessier D.J., Thresher J., Komalavilas P., Brophy C.M J. Am. Coll. Surg. 2005, Vol 201 (1):30-36
2005
Pre-B-cell colony-enhancing factor is a secreted cytokine-like protein from the human amniotic epithelium. Ognjanovic S, Ku TL, Bryant-Greenwood GD. Am J Obstet Gynecol. 2005 Jul;193(1):273-82
2005
Western Blotting
A high-affinity reversible protein stain for Western blots Antharavally B.S., Carter, B., Bell, P.A., Mallia K. Analytical Biochemistry 2004,Vol 329:276-280
2004
Biochemical analysis of GABA receptor subunits alpha 1, alpha 5, beta1 beta2 in the hippocampus of patients with Alzheimer's disease neurophathology Rissman, R.A., Mishizen-Eberz A.J. N.,Wolfe, C.B.B., DeBlas A.L., Miralles C.P., Ikonomovic M.D., armstrong D.M. Neuroscience 120 (2003) 295-705
2003
Western Blotting
Proteomics reveals protein profile changes in doxorubicin treated MCF7 human breast cancer cells Cheng S.T., Pan T, L., Tsai Y.Ch, Huang C. M. Cancer letters 2002. vol 181:95-107
2002
Towards proteome-wide production of monoclonal antibody by phage display. Bin Liu, Lan Huang, Carina Sihlbom, Al Burlingame and James D. Marks. J Mol Biol. 2002 Feb 1;315(5):1063-73
2002
Mass Spectrometry Sample Prep
Characterization of retinoic acid receptor-deficient keratinocytes. Goyette Philippe; Chen Chang Feng; Wang Wei; Seguin Francois; Lohnes David(a) Journal of Biological Chemistry v 275 pg 16497-16505 June 2, 2000
2000
Protection of renal inner medullary epithelial cells from apoptosis by hypertonic stress-induced p53 activation Dmitrieva Natalia(a); Kultz Dietmar; Michea Luis; Ferraris Joan; Burg Maurice ; Journal of Biological Chemistry v 275, pg 18243-18247 June 16, 2000 Journal of Biological Chemistry v 275, pg 18243-18247 June 16, 2000
2000
I have just received the 0.2um Immobilon-PSQ membrane. Does the membrane require any special handling procedure?
The Immobilon-PSQ can be handled in the same way as Immobilon-P. Prewetting in methanol and exchanging in MilliQ water are the only requirements prior to blotting.
What effect will SDS have on the New Immobilon PSQ 0.2 um membrane?
SDS interferes with the binding of protein to PVDF during transfer. Because this membrane is thicker than Immobilon P, there is a high probability that the protein will stick to the membrane. Because of the tighter pore size and increased thickness, the residence time of the protein within the membrane will favor its binding to the PVDF. If the protein is not retained well on the 0.2 um membrane, reducing the voltage or current may help as would lowering the SDS concentration ( only as a secondary step if reducing current/voltage does not work).
What are Millipore's suggestions to reduce blow-through with the new 0.2um Immobilon PSQ?
If blow-through is an issue, modify transfer conditions by reducing the voltage or current.
How much protein do I need for sequencing?
For most purposes, if the protein is visible by staining with Coomassie brilliant blue R, then there is enough protein present for sequence analysis. Check with your protein sequencing facility on the minimum requirements for their instrumentation.
Can the 0.2 um Immobilon PSQ membrane be used for Immunodetection ?
Immobilon- PSQ is recommended for transfering and detecting proteins of molecular weight less than 20 kD.
Can I use ECL with Immobilon-PSQ ?
Yes, standard chemilumniescent detection can be used with Immobilon-PSQ. Conditions used for the Immobilon-P will most likely require some optimization when switching to Immobilon-PSQ. Since the binding capacity of the Immobilon-PSQ is greater the mass of the blocking agent must be increased as well as the incubation time. You will also need to increase the wash time to ensure that the unbound anitbody is washed out of the tighter pores. Antibody concentrations may also need to be increased because the molecules are more likely to be distributed deeper into the pores. However modifying the blocking and wash steps as recommended may make adjusment of the antibody concentration unnecessary.
Can I use Immobilon-PSQ for all protein blotting applications?
The Immobilon-PSQ membrane should not be used as a replacement for the Immobilon P unless Immobilon-P is not working in a particular protein transfer system. Immobilon-P has demonstrated superior capabilities in applications such as standard immunodetection, rapid immunodetection, standard ECL, rapid ECL and transillumination. The use Immobilon-PSQ membrane has been shown to be most applicable in the in immunoblotting of relatively small molecular weight proteins (less than 20,000 daltons).
Should I prewet MultiScreen Immobilon-P plates before use?
Yes. Use 15 ul of 70% ethanol or methanol to prewet the Immobilon P membrane. This membrane is hydrophobic and requires a prewet to allow liquid to pass through the membrane.
What is the protein binding capacity of Immobilon-P?
The protein (BSA) binding capacity for the Immobilon P membrane is 131 micrograms per sq. cm. of membrane.
C3117Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications.
Millipore offers four different Immobilon® PVDF (Polyvinylidene Difluoride) membranes, each optimized for different protein blotting applications. We also now offer blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper.
Immobilon® membranes provide a number of advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.
Immobilon®-P Membrane
0.45 µm pore size
Recommended for most western blotting, especially proteins >20 kDa
Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity
Immobilon®-E Membrane
The only PVDF membrane that wets out in water—no alcohol required
0.45 µm pore size ideal for most western blotting applications
Save time and reduce waste by eliminating the alcohol pre-wet step
Immobilon-PSQ Membrane
0.2 µm pore size and a large internal structure
Higher protein adsorption and sequencing yields than other
membranes
Recommended for blotting proteins <20 kDa
Prevents blow-through of low molecular weight proteins
Immobilon-FL Membrane
The first transfer membrane optimized for fluorescence applications
Extremely low background improves sensitivity of all fluorescence detection protocols
Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
Ideal for multiplexing and chemifluorescence applications
Immobilon® NOW Rolls & Dispenser
Convenience of cut sheets with the flexibility of rolls, offered for each Immobilon® PVDF membrane
Mini or midi size with a single cut
No wasted membrane
More compact package saves space in your lab
Blotting Sandwiches
When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon®-E or Immobilon®-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper.
Blotting Filter Paper
Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.
Performance
Rapid Immunodetection Protocol with Immobilon-P Membrane
Step
Standard Immunodetection
Rapid Immunodetection
1. Block the membrane
1 h
None
2. Incubate with primary antibody
1 h
1 h
3. Wash the membrane
3 x 10 min
3 x 5 min
4. Incubate with secondary antibody
1 h
30 min
5. Wash the membrane
3 x 10 min
3 x 5 min
6. Add substrate
5 min
5 min
Total time
4 h 5 min
2 h 5 min
Immobilon-PSQ Membrane Prevents Blow-through
Molecular weight standards (lanes 1, 3, 5, 7) and calf liver lysate (lanes 2, 4, 6, 8) were transferred to Immobilon-P or Immobilon-PSQ membranes. A sheet of Immobilon-PSQ was placed behind the primary membranes to capture proteins that passed through (lanes 5 and 6 behind Immobilon-P; lanes 7 and 8 behind Immobilon-PSQ).
Immobilon-FL Membrane is Optimized for Fluorescence
Actin-tubulin multiplex detection on Immobilon-FL membrane. Rabbit muscle actin (red) was detected using rabbit anti-actin 1oAB and QDot® 655 goat anti-rabbit 2oAB. Porcine brain tubulin (green) was detected using mouse anti-tubulin 1oAB and QDot 565 goat anti-mouse 2oAB. Sensitivities down to 7 ng were observed for both analytes. Data provided by Quantum Dot Corporation.
Immobilon® PVDF membranes are offered in three types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.
Features & Benefits
Won’t crack, curl or fracture when cut
Low background
Superior staining capabilities
Can be reprobed multiple times
Applications
Western Blotting, Dot Blotting, Protein Sequencing; Compatible with Radioactive, Chromogenic, Chemiluminescent, Fluorescent, and Chemifluorescent Detection
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