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17-20000 Magna ChIP™ G Tissue Kit

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22 ChIP assays from tissue samples.  
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      Replacement Information
      Catalogue Number17-20000
      Trade Name
      • Magna ChIP
      DescriptionMagna ChIP™ G Tissue Kit
      OverviewChromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.

      Historically, ChIP protocols have been geared towards homogenous populations of cell culture. In contrast, tissue samples are more complex and the cells are heterogeneous thus making ChIP analysis less amenable. The Magna ChIP™ G Tissue Kit provides the tools necessary to obtain repeatable, reliable, and site specific tissue biopsies. Microdissected functionally related populations of cells within a heterogeneous tissue can thus be analyzed with ease, precision and certainty.
      Materials Required but Not DeliveredCells, stimulated or treated as desired
      Antibody for chromatin immunoprecipitation
      37% Formaldehyde
      Taq DNA polymerase and dNTPs
      Master Mix for qPCR
      DNase and RNase-free sterile H2O
      Quant-iT™ dsDNA HS Assay Kit, (Life Technologies catalog #: Q32851)
      O.C.T. – Optimal Cutting Temperature compound
      RNase A
      Magna Grip™ Rack 8 well (Cat.#20-400) or similar magnetic rack
      Vortex mixer
      Rotating wheel/platform
      Thermomixer or Hybridization Oven
      Variable temperature water bath or incubator
      Variable volume (5-1000 l) pipettors + tips
      Microfuge & PCR tubes
      Thermal cycler
      Filter-tip pipette tips
      Product Information
      • Magnetic Protein G beads
      • ChIP Dilution Buffer
      • Low Salt Wash Buffer
      • High Salt Wash Buffer
      • LiCl Wash Buffer
      • TE Buffer
      • Tissue Lysis Buffer
      • 10X Tissue Stabililizing Solution
      • ChIP Elution buffer (w/o Proteinase K)
      • 10X Glycine
      • 10X PBS
      • 1mm Micro-dissection punch
      • Spin Filters
      • Collection Tubes
      • Bind Reagent A
      • Wash Reagent B
      • Elution Reagent C
      • Protease Inhibitor Cocktail II
      • Proteinase K
      PresentationTwo modules containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions from tissue samples.
      Quality LevelMQ100
      ApplicationThe Magna ChIP G Tissue Kit provides the tools necessary to obtain repeatable, reliable & site specific tissue biopsies.
      Key Applications
      • Sample Prep
      Biological Information
      Analytes Available
      • Protein G
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when used and stored as directed.
      Packaging Information
      Material Size22 ChIP assays from tissue samples.
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalogue Number GTIN
      17-20000 04053252010972


      Magna ChIP™ G Tissue Kit SDS


      Safety Data Sheet (SDS) 


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