07-687
Anti-Cyclin E Antibody
Upstate®, from rabbit
Synonym(s):
Anti-CCNE, Anti-pCCNE1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Cyclin E Antibody, Upstate®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CCNE1(898)
Analysis Note
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from 3T3/A31 cells
Application
Detect Cyclin E with Anti-Cyclin E Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP, WB & IHC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Biochem/physiol Actions
Cyclin E
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
Mr 52kDa
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 378-396 (GVLTPPHSSKKQSSEQETE) of rat Cyclin E
Other Notes
Replaces: 04-223
Physical form
ImmunoAffinity Purified
PBS containing 0.1% sodium azide and 0.2% gelatin.
Preparation Note
stable for 1 year at 4°C from date of shipment
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Shigeki Sekine, Pedro J A Gutierrez, Billy Yu-Ang Lan, Sandy Feng, Matthias Hebrok
Hepatology null
Cyclin A recruits p33cdk2 to the cellular transcription factor DRTF1.
Bandara, L R, et al.
Journal of Cell Science. Supplement, 16, 77-85 (1992)
Ssang-Taek Lim et al.
Molecular cell, 29(1), 9-22 (2008-01-22)
FAK is known as an integrin- and growth factor-associated tyrosine kinase promoting cell motility. Here we show that, during mouse development, FAK inactivation results in p53- and p21-dependent mesodermal cell growth arrest. Reconstitution of primary FAK-/-p21-/- fibroblasts revealed that FAK
Cell cycle regulation in mouse heart during embryonic and postnatal stages.
Aiko Ikenishi,Hitomi Okayama,Noriko Iwamoto,Satoshi Yoshitome,Shoji Tane,Kazuomi Nakamura et al.
Development, Growth & Differentiation null
Philipp Schuler et al.
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The analysis of the non-exchangeable hydrogen isotope ratio (δ2 Hne ) in carbohydrates is mostly limited to the structural component cellulose, while simple high-throughput methods for δ2 Hne values of non-structural carbohydrates (NSC) such as sugar and starch do not
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 07-687 | 04053252511806 |
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