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1.11350

Bactident™ Kovac′s Indole reagent

Available in a practical dropper bottle, for Escherichia coli

Synonym(s):

4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac, Indole reagent, Indole test reagent

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About This Item

NACRES:
NA.84
UNSPSC Code:
41106200
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Quality Segment

100

description

Bactident™ E. coli 1.13303

form

liquid (ready-to-use)

shelf life

210 days

feature

ready-to-use

composition

(n-Butanol; Hydrochloric acid; 4 dimethylaminobenzaldehyde)

packaging

pkg of (1 x 30 ml, Plastic bottle)

technique(s)

microbe id | specific enzyme detection: suitable

pH

<1 (20 °C in H2O)

transition temp

flash point 36 °C

density

0.92 g/cm3 at 20 °C

application(s)

environmental
food and beverages

storage temp.

2-8°C

suitability

Enterobacter spp., Escherichia coli

General description

Kovac′s reagent is a biochemical reagent composed of n-Butanol, hydrochloric acid, and 4-dimethylaminobenzaldehyde. It is used for the detection of indole producing microorganisms. Tryptophan is abundant in trypticalle digested peptone. Some microorganisms can cleave tryptophan to give pyruvic acid, ammonia, and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a color reaction with 4 dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.

Application

Bactident™ Kovac′s Indole reagent is used for the detection of indole producing microorganisms in food and beverages, and environmental samples. This is suitable for the identification of E. coli.

Analysis Note

Indole formation
- Escherichia coli ATCC 25922 (WDCM 00013):
+
- Proteus vulgaris ATCC 8427: +
- Enterobacter aerogenes ATCC 13048: -
Incubation: 24 hrs.; 35 °C; aerobic.
Reference: DEV-Tryptophan broth.

Other Notes

Replaces: 1.11350.0007

Legal Information

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1 - Skin Sens. 1 - STOT SE 3

Storage Class

3 - Flammable liquids

flash_point_f

96.8 °F

flash_point_c

36 °C

target_organs

Central nervous system, Respiratory system

Certificates of Analysis (COA)

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