1.15934
Eosin B (bluish) (C.I.45400)
for microscopy Certistain®
Synonym(s):
Eosin B (bluish) (C.I.45400), Dibromodinitrofluorescein sodium
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About This Item
Empirical Formula (Hill Notation):
C20H6Br2N2Na2O9
CAS Number:
Molecular Weight:
624.06
UNSPSC Code:
41116130
EC Index Number:
208-943-1
MDL number:
Quality Segment
form
solid
composition
Dye, ≥85% spectrophotometric assay
pH
6.2 (25 °C, 10 g/L in H2O)
solubility
300 g/L
bulk density
680 kg/m3
εmax
800-1000 at 517-525 nm in water at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-30°C
SMILES string
[Na+].[Na+].Brc1c2c(cc(c1[O-])[N+](=O)[O-])C(=C4C(=C(C(=O)C(=C4)[N+](=O)[O-])Br)O2)c3c(cccc3)C(=O)[O-]
InChI
1S/C20H8Br2N2O9.2Na/c21-14-16(25)11(23(29)30)5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(24(31)32)17(26)15(22)19(10)33-18(9)14;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
InChI key
GYYTYUGGVBYJHE-UHFFFAOYSA-L
General description
Eosin B (C.I. 45400) for microscopy Certistain®, is a dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin B staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear dark blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances pink to red. As the dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Analysis Note
Dye content (gravimetrically): ≥ 85 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 517 - 525 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water): 800 - 1000
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 517 - 525 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water): 800 - 1000
TLC-Test: passes test
Loss on drying (110 °C): ≤ 8 %
Suitability for microscopy: passes test
Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_c
Not applicable
flash_point_f
Not applicable
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