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Merck

16-104

Anti-Phosphotyrosine Antibody, clone 4G10®, FITC conjugate

clone 4G10®, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-Phosphotyrosine Antibody, clone 4G10®, FITC conjugate, clone 4G10®, Upstate®, from mouse

biological source

mouse

conjugate

FITC conjugate

antibody product type

primary antibodies

clone

4G10®, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr)

Quality Level

Gene Information

human ... PID1(55022)

Analysis Note

Control
Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells
routinely evaluated in immunocytochemistry to detect tyrosine phosphorylated proteins in EGF-stimulated human A431 cells

Application

Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, FITC conjugate validated for use in IC.
For use in applications in which a FITC conjugate is advantageous, such as ICC.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

Biochem/physiol Actions

Recognizes tyrosine-phosphorylated proteins from all species.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-phosphotyrosine antibody (05-321), cross-linked to fluorescein isothiocyanate (FITC).

Immunogen

Phosphotyramine-KLH.

Physical form

PBS containing 0.05% sodium azide
Protein G Purified

Preparation Note

Stable for 9 months at 4°C

Legal Information

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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David Vandael et al.
Nature protocols, 16(6), 2947-2967 (2021-05-16)
Rigorous investigation of synaptic transmission requires analysis of unitary synaptic events by simultaneous recording from presynaptic terminals and postsynaptic target neurons. However, this has been achieved at only a limited number of model synapses, including the squid giant synapse and
B Josh Lane et al.
PLoS pathogens, 4(3), e1000014-e1000014 (2008-04-04)
Chlamydia trachomatis attachment to cells induces the secretion of the elementary body-associated protein TARP (Translocated Actin Recruiting Protein). TARP crosses the plasma membrane where it is immediately phosphorylated at tyrosine residues by unknown host kinases. The Rac GTPase is also
Xiaomin Zhang et al.
Neuron, 107(6), 1212-1225 (2020-08-09)
Dentate gyrus granule cells (GCs) connect the entorhinal cortex to the hippocampal CA3 region, but how they process spatial information remains enigmatic. To examine the role of GCs in spatial coding, we measured excitatory postsynaptic potentials (EPSPs) and action potentials
José Ursic-Bedoya et al.
Molecular metabolism, 60, 101483-101483 (2022-04-04)
Fibroblast growth factor 19 (FGF19) is a hormone with pleiotropic metabolic functions, leading to ongoing development of analogues for treatment of metabolic disorders. On the other hand, FGF19 is overexpressed in a sub-group of hepatocellular carcinoma (HCC) patients and has
Experimental inoculation of juvenile rhesus macaques with primate enteric caliciviruses.
Sestak, K; Feely, S; Fey, B; Dufour, J; Hargitt, E; Alvarez, X; Pahar, B; Gregoricus et al.
Testing null

Global Trade Item Number

SKUGTIN
16-10404053252473159
SCC07204055977179606

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