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Merck

537060

Propidium Iodide Solution

A convenient form of propidium iodide useful for flow cytometry studies.

Synonym(s):

Propidium Iodide Solution

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About This Item

Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
25535-16-4
Molecular Weight:
668.39
UNSPSC Code:
12352200
NACRES:
NA.47
MDL number:
MFCD00011921

Product Name

Propidium Iodide Solution, A convenient form of propidium iodide useful for flow cytometry studies.

SMILES string

[I-].I.[N+](CCCN1C2=CC(=N)C=CC2=C3C(=C1c4ccccc4)C=C(C=C3)N)(CC)(CC)C

InChI

1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1

InChI key

XJMOSONTPMZWPB-UHFFFAOYSA-M

description

RTECS - SF7949600

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

input

sample type intact cells

fluorescence

λex 536 nm
λem 617 nm

shipped in

ambient

storage temp.

2-8°C

Quality Level

100

Related Categories

Biochem/physiol Actions

Product does not compete with ATP.
Reversible: no
Cell permeable: no
Primary Target
Membrane impermeable DNA intercalator

Disclaimer

Toxicity: Standard Handling (A)

General description

Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.

Other Notes

Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.

Physical form

50 µg/ml in PBS, pH 7.4.

Preparation Note

Following initial use, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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