Anti-CYP2J2 Antibody, clone 6-5-20-8

Cytochrome P450 2J2 (UniProt: P51589; also known as EC:1.14.14.1, Arachidonic acid epoxygenase, CYPIIJ2) is encoded by the CYP2J2 gene (Gene ID: 1573) in human. Cytochrome P450 2J2 is an endoplasmic reticulum microsomal enzyme that metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. It is responsible for the epoxidation of endogenous cardiac arachidonic acid pools. It is highly expressed in heart and is present at lower levels in liver, ileum, jejunum, colon, and kidney. It is reported to activate the nuclear peroxisome proliferator-activated receptor alpha (PPAR alpha) and hence plays a role in lipid metabolism and inflammation. Overexpression of CYP2J2 in vivo is shown to reverse several pathological processes in animals, including hypertension, other cardiovascular pathologies, and insulin resistance.

~52 kDa observed; 57.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

A full-length recombinant human cytochrome P450 2J2.

Anti-CYP2J2, clone 6-5-20-8, Cat. No. MABS1242, is a mouse monoclonal antibody that targets cytochrome P450 2J2 and is tested for use in Inhibition Assay and Western Blotting.

Inhibition Analysis: A representative lot detected CYP2J2 in Inhibition applications (Xiao, Y.F., et. al. (2004). Mol Pharmacol. 66(6):1607-16).

Western Blotting Analysis: A representative lot detected CYP2J2 in Western Blotting applications (Xiao, Y.F., et. al. (2004). Mol Pharmacol. 66(6):1607-16).

Research Category
Signaling

Clone 6-5-20-8 detects cytochrome p450 2J2 in human and murine species.

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Evaluated by Western Blotting in mouse heart tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CYP2J2 in 20 µg of mouse heart tissue lysate.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.