A1602

Amyloglucosidase from Aspergillus niger

Name: Amyloglucosidase from <I>Aspergillus niger</I>
Brand: SIGMA

ammonium sulfate suspension, ≥40 units/mg protein

Synonym(s):

1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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About This Item

CAS Number:

9032-08-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.2.1.3 (BRENDA, IUBMB)

MDL number:

MFCD00081350

Product Name

Amyloglucosidase from Aspergillus niger, ammonium sulfate suspension, ≥40 units/mg protein

form

ammonium sulfate suspension

specific activity

≥40 units/mg protein

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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Related Categories

Glycobiology Profiling Tools

Protein Biology

Proteins & Enzymes

Application

Amyloglucosidase from Aspergillus niger is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Sigma, has been used to hydrolyze glycogen into glucose monomers in order to study lipid accumulation in skeletal muscle .

Amyloglucosidase from Aspergillus niger has been used to liberate free glucose in the Belgica antarctica larva homogenate. It has also been used in digestive enzyme mixture for starch digestion.

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides .

General description

Amyloglucosidase, a disaccharide enzyme is a member of the alpha-glucosidase type. It is produced by several species of Aspergillus genus.

Other Notes

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH approx. 6.0

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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A method for routine measurements of total sugar and starch content in woody plant tissues

Chow PS and Landhausser SM

Tree physiology, 24(10), 1129-1136 (2004)

Biodiversity: New Leads for the Pharmaceutical and Agrochemical Industries, 183-183 (2000)

Sustained effect of glucagon on body weight and blood glucose: Assessed by continuous glucose monitoring in diabetic rats.

Christina Pedersen et al.

PloS one, 13(3), e0194468-e0194468 (2018-03-21)

Insulin is a vital part of diabetes treatment, whereas glucagon is primarily used to treat insulin-induced hypoglycemia. However, glucagon is suggested to have a central role in the regulation of body weight, which would be beneficial for diabetic patients. Since

Survival and energetic costs of repeated cold exposure in the Antarctic midge, Belgica antarctica: a comparison between frozen and supercooled larvae

Teets NM, et al.

The Journal of Experimental Biology, 214(5), 806-814 (2011)

Low-carbohydrate diet induces metabolic depression: a possible mechanism to conserve glycogen.

Hugh S Winwood-Smith et al.

American journal of physiology. Regulatory, integrative and comparative physiology, 313(4), R347-R356 (2017-07-14)

Long-term studies have found that low-carbohydrate diets are more effective for weight loss than calorie-restricted diets in the short term but equally or only marginally more effective in the long term. Low-carbohydrate diets have been linked to reduced glycogen stores

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