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About This Item
Empirical Formula (Hill Notation):
C8H17NO3S
CAS Number:
Molecular Weight:
207.29
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
203-115-6
Beilstein/REAXYS Number:
2967601
MDL number:
SMILES string
OS(=O)(=O)CCNC1CCCCC1
InChI
1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)
InChI key
MKWKNSIESPFAQN-UHFFFAOYSA-N
assay
≥99.0% (titration)
form
crystalline
pH
3.0-5.0 (103.6 g/L)
useful pH range
8.6-10.0
pKa (25 °C)
9.3
solubility
water: 0.6 g/25 mL, clear, colorless
application(s)
diagnostic assay manufacturing
Quality Level
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General description
CHES (2-(N-cyclohexylamino)ethanesulphonic acid) is regarded as Good′s buffers for its self-buffering and biocompatible feature. It is used in protein stabilization and does not interact with metals.
Application
CHES has been used:
- as a buffer in mannanase activity assay of antarctic fungal isolates
- as a buffer for DNA digestion and labeling of 2′-deoxyadenosine 3′-monophosphate
- as a component of Wilson and Horne extraction buffer for characterization of rat plasma enzymes
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Determination of folate in some cereals and commercial cereal-grain products consumed in Poland using trienzyme extraction and high-performance liquid chromatography methods
Gujska E and Kuncewicz A
European Food Research and Technology, 221(1-2), 208-213 (2005)
Hemicellulase activity of antarctic microfungi
Bradner JR, et al.
Journal of Applied Microbiology, 87(3), 366-370 (1999)
Good's buffers as a basis for developing self-buffering and biocompatible ionic liquids for biological research
Taha M, et al.
Green Chemistry, 16(6), 3149-3159 (2014)
Detection of Bulky Endogenous Oxidative DNA Lesions Derived from 8, 5?-Cyclo-2?-deoxyadenosine by 32P-Postlabeling Assay
Zhou GD and Moorthy B
Current Protocols in Toxicology / Editorial Board, Mahin D. Maines (editor-in-chief) ... [Et al.], 64(1), 17-17 (2015)
P Doble et al.
Electrophoresis, 19(12), 2257-2261 (1998-10-07)
The suitability of relatively slow (low absolute value of mobility) coanionic buffers in background electrolytes (BGEs) for indirect photometric detection of anions by capillary electrophoresis was investigated. As a model system, 2-(cyclohexylamino)ethanesulfonic acid (CHES) was used to buffer the indirect
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