C7805

Anti-Collagen Type III (COL3A1) Antibody

Name: Anti-Collagen Type III (COL3A1) Antibody
Brand: SIGMA

mouse monoclonal, FH-7A

Synonym(s):

Anti-Collagen III antibody

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All Photos(3)

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00239753

Product Name

Monoclonal Anti-Collagen, Type III antibody produced in mouse, clone FH-7A, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

FH-7A, monoclonal

mol wt

antigen 70 kDa

contains

15 mM sodium azide

species reactivity

human, rat

technique(s)

dot blot: suitable
immunohistochemistry (frozen sections): 1-4,000 using frozen sections of human skin
indirect ELISA: suitable
western blot: suitable using a denatured-reduced preparation

isotype

IgG1

UniProt accession no.

P02461

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... COL3A1(1281)
rat ... Col3a1(84032)

Application

Monoclonal Anti-Collagen, Type III antibody produced in mouse has also been used in enzyme linked immunosorbent assay (ELISA) and immunohistochemistry.

Monoclonal anti-collagen type III antibody has been used in immunofluorescence and western blot.

Biochem/physiol Actions

Monoclonal Anti-Collagen Type III reacts specifically with native and denatured collagen type III. It does not recognize collagen types I, II, IV, V, VI, and X.

This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Monoclonal anti-collagen type III antibody can be used to determine the interstitial collagen I and III content in total protein homogenates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type III is a 300 kD molecule that belongs to fibrillar collagens family found mainly in skin, blood vessels, liver, placenta, tongue and thymus. This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Monoclonal anti-collagen type III antibody can be used to determine the interstitial collagen I and III content in total protein homogenates. It is also used to study the immunolocalization of collagen type III in normal and neoplastic tissue. Mouse monoclonal anti-collagen type III antibody reacts specifically with native and denatured collagen type III but not with collagen types I, II, IV, V, VI, and X.

Monoclonal Anti-Collagen Type III (mouse IgG1 isotype) is derived from the FH-7A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human collagen type III.

Immunogen

human collagen type III

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000484681

0000443857

0000439625

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