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Merck

DLD-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90102540, human colon, Epithelial

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About This Item

UNSPSC Code:
41106514
Biological source:
human colon
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
2n = 46, pseudodiploid
Morphology:
Epithelial

Key Documents

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biological source

human colon

packaging

tube of 5 μg 90102540-DNA-5UG, pkg of vial of cells 90102540-1VL

growth mode

Adherent

karyotype

2n = 46, pseudodiploid

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

DLD-1 cell line has been used to express shRNA oligos directed against coronin 2A (CRN5) and to study the role of CRN5 in tumor cell migration. It has also been used to determine the efficiency of antibody-coupled short interfering RNA (siRNA) for in vivo mRNA knockdown.

Biochem/physiol Actions

Derived from human colorectal adenocarcinoma. The cells have been used in the study of polar solvents on cell characteristics.The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.
Human colon adenocarcinoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin, 0.02% EDTA.

Other Notes

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