G9269

Anti-Glial Fibrillary Acidic Protein Antibody

Name: Anti-Glial Fibrillary Acidic Protein Antibody
Brand: SIGMA

rabbit polyclonal

Synonym(s):

GFAP Antibody Sigma - Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, Gfap Antibody Sigma, Anti-GFAP

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00145904

Product Name

Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, human

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using brain sections
microarray: suitable
western blot: 1:500 using brain extract

UniProt accession no.

P14136

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Application

Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit is suitable for:

immunohistochemical staining at a working dilution of 1:400 using rat brain sections
immunohistochemical staining of human brain tumor tissue specimens to identify the cells expressing AQP4 (Aquaporin-4)
immunofluorescence using brain section from mice

The antibody is suitable for immunohistochemistry at a working dilution of 1:80 using alcohol- and formalin-fixed, paraffin-embedded sections of human or animal brain tissue. It is also suitable for indirect immunoblotting at a working dilution of 1:500 using human brain extract blot and microarray.

Biochem/physiol Actions

Glial fibrillary acidic protein may be used as a marker for distinguishing astrocytes from other glial cells during development of the central nervous system. GFAP is responsible for maintaining the structure of glial cells. It also offers mechanical strength and support to the surrounding neurons and blood brain barrier. Defects in this gene causes Alexander disease, indicated by Rosenthal fibers deposition. It is a rare disorder of astrocytes in the central nervous system. Increased GFAP expression indicates astrogliosis, a sign of neurodegeneration and neuroinflammation.

The product reacts specifically with GFAP and labels astrocytes, glial cells and glial cell derived tumors in immunohistochemical staining. The antibody reacts with glial specific antigen in alcohol or formalin-fixed, paraffin-embedded human or animal tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The GFAP (glial fibrillary acidic protein) gene encodes an intermediate protein filament-III (50kDa). It is localized particularly to the astrocytes and non-myelinating Schwann cells of the central and peripheral nervous system, respectively.

Immunogen

GFAP from human brain

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

Store at 2-8 °C up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000488151

0000458268

0000426879

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Aquaporin-4 expression is increased in oedematous human brain tumours.

S Saadoun et al.

Journal of neurology, neurosurgery, and psychiatry, 72(2), 262-265 (2002-01-18)

Aquaporin-4 (AQP4) is a highly conserved water channel protein. In rats, AQP4 is expressed in astrocyte foot processes and is important in brain water homeostasis. AQP4 expression has not been investigated in non-neoplastic human brain or oedematous brain tumours, where

Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker

Yang Z and Wang K K

Trends in Neurosciences, 38(6), 364-374 (2015)

Obesity and diabetes cause cognitive dysfunction in the absence of accelerated β-amyloid deposition in a novel murine model of mixed or vascular dementia.

Dana M Niedowicz et al.

Acta neuropathologica communications, 2, 64-64 (2014-06-12)

Mid-life obesity and type 2 diabetes mellitus (T2DM) confer a modest, increased risk for Alzheimer's disease (AD), though the underlying mechanisms are unknown. We have created a novel mouse model that recapitulates features of T2DM and AD by crossing morbidly

Effects of a polymorphism in the GFAP promoter on the age of onset and ambulatory disability in late-onset Alexander disease.

Tomokatsu Yoshida et al.

Journal of human genetics, 58(9), 635-638 (2013-08-02)

Alexander disease (AxD) is a rare neurodegenerative disorder. Most patients with AxD have a de novo dominant missense mutation in the glial fibrillary acidic protein (GFAP) gene. Patients with late-onset AxD exhibit a more variable onset and severity than patients

Induction of glial fibrillary acidic protein expression in astrocytes by nitric oxide

Brahmachari S, et al.

The Journal of Neuroscience, 26(18), 4930-4939 (2006)

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