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HT501850

Formalin solution, neutral buffered, 10%

histological tissue fixative

Synonym(s):

10% NBF, 10% Neutral Buffered Formalin, Formaldehyde, Methanal

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About This Item

UNSPSC Code:
12171500
PubChem Substance ID:
24895892
NACRES:
NA.47
MDL number:
MFCD00003274
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biological source

synthetic

Quality Level

200

concentration

10% formalin (approx. 4% formaldehyde)

color

colorless

pH

6.90-7.10

density

1.080 g/cm3

application(s)

hematology
histology

storage temp.

room temp

SMILES string

[O-]#[C+H2]

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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General description

10% Neutral Buffered Formalin is the most used fixative throughout the world for light microscopy. Formalin solution is the commercially obtained solution containing 37% w/w (40% w/v) of formaldehyde in water. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.

Application

Popular general purpose histological fixative.
Formalin solution (neutral buffered, 10%) has been popularly used as a general-purpose histological fixative to fix tissue samples.
  • It effectively preserves the cellular and tissue morphology, maintaining the structural integrity required for microscopic examination.
  • It may be used as a reagent for the primary fixation of tissues for diagnostic applications of an immunoperoxidase method in hematopathology.
  • It is used for routine histological staining, providing clear and detailed images of cellular structures.

Biochem/physiol Actions

Formaldehyde is both a non-coagulant and an additive fixative. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins.

Features and Benefits

  • 10% NBF is well-suited for large throughput laboratories.
  • It requires a relatively short period of fixation.
  • It can be used for the long-term storage of tissue.

Physical form

Neutral buffered solution

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

wgk

WGK 3

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

185.0 °F

flash_point_c

85 °C

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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ8273
MKCZ7680
MKCZ6233
MKCZ2854
MKCS5167

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