L3540
Lowry Reagent
Synonym(s):
Copper(II) tartrate Mix
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
Quality Level
General description
The Lowry method has been for decades the procedure of choice for quantitation of soluble proteins due to its sensitivity, simplicity, and precision. Peterson′s modification of the micro Lowry method is simple, rapid, objective and more generally applicable. In this procedure sodium dodecyl sulfate (SDS) is mixed with Lowry reagent to enable the dissolution of relatively insoluble lipoproteins.
Application
Lowry Reagent has been used to quantify the protein content in extracellular polymeric substance (EPS). It has also been used to quantify total protein and mitochondrial protein in Drosophila melanogaster.
For protein determination. Utilizes SDS to facilitate dissolution of relatively insoluble lipoporoteins.
Preparation Note
Reconstitute to 40 mL with deionized water.
Other Notes
Dry powder mixture containing Lowry reagent with sodium dodecyl sulfate.
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Corr. 1B
Storage Class
4.1B - Flammable solid hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Ginger extract inhibits biofilm formation by Pseudomonas aeruginosa PA14
Kim HS and Park HD
PLoS ONE, 8(9), e76106-e76106 (2013)
Environmental and genetic perturbations reveal different networks of metabolic regulation
Greenberg A, et al.
Molecular Systems Biology, 7(1), 563-563 (2011)
A simplification of the protein assay method of Lowry et al. which is more generally applicable
Peterson GL
Analytical Biochemistry, 83(2), 346-356 (1977)