M8662
Mineral oil
PCR Reagent
Synonym(s):
Mineral oil for PCR
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About This Item
technique(s):
PCR: suitable
Product Name
Mineral oil, PCR Reagent
Quality Level
InChI key
AEOVEGJBKQQFOP-DDVLFWKVSA-L
form
liquid
packaging
vial of 1.5 mL (Total volume 7.5 mL (5 vials))
technique(s)
PCR: suitable
color
colorless
refractive index
n20/D 1.467 (lit.)
density
0.84 g/mL at 25 °C (lit.)
foreign activity
DNase, RNase, protease, none detected
storage temp.
room temp
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Application
Mineral oil has been used:
- for routine PCR amplifications
- to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification
- to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform
Features and Benefits
- Provided in a convenient 5 × 1.5 mL (1 vial) pack size
- Tested for the absence of DNase, RNase, and protease.
General description
Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
No data available
flash_point_c
No data available
ppe
Eyeshields, Gloves
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Ivan K Dimov et al.
Nature communications, 5, 3451-3451 (2014-03-29)
Discriminating cellular heterogeneity is important for understanding cellular physiology. However, it is limited by the technical difficulties of single-cell measurements. Here we develop a two-stage system to determine cellular heterogeneity. In the first stage, we perform multiplex single-cell RNA cytometry
Hartmut Kaiser et al.
Plant physiology, 143(2), 1068-1077 (2006-12-13)
The response of stomata to a reduction of air humidity is composed of a hydropassive opening followed by active closure. Whereas the mechanisms behind the hydropassive opening are largely understood, the location and physiological basis of the sensing mechanisms leading
Hsin-I Jen et al.
eLife, 8 (2019-04-30)
The mammalian cochlea loses its ability to regenerate new hair cells prior to the onset of hearing. In contrast, the adult vestibular system can produce new hair cells in response to damage, or by reprogramming of supporting cells with the
Decagram scale production of deuterated mineral oil and polydecene as solvents for polymer studies in neutron scattering
Klenner MA, et al.
Open Journal of Polymer Chemistry, 11(31), 4986-4994 (2020)
Neus Bota-Rabassedas et al.
Cell reports, 35(3), 109009-109009 (2021-04-22)
Cancer cells function as primary architects of the tumor microenvironment. However, the molecular features of cancer cells that govern stromal cell phenotypes remain unclear. Here, we show that cancer-associated fibroblast (CAF) heterogeneity is driven by lung adenocarcinoma (LUAD) cells at
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