P5412
o-Phenylenediamine
peroxidase substrate, chromogenic, tablet
Synonym(s):
1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD
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About This Item
Empirical Formula (Hill Notation):
C6H8N2
CAS Number:
Molecular Weight:
108.14
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
202-430-6
MDL number:
Beilstein/REAXYS Number:
606074
Product Name
o-Phenylenediamine, tablet, 20 mg substrate per tablet
vapor density
3.7 (vs air)
Quality Level
vapor pressure
0.01 mmHg ( 25 °C)
form
tablet
bp
256-258 °C
mp
100-102 °C
fluorescence
λex 337 nm; λem 417 nm (after derivatization)
storage temp.
2-8°C
SMILES string
Nc1ccccc1N
InChI
1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2
InChI key
GEYOCULIXLDCMW-UHFFFAOYSA-N
Application
o-Phenylenediamine has been used to detect transforming growth factor (TGF-β) and to measure anti-cTfRMAb-ScFv fusion protein using enzyme linked immunosorbent assay (ELISA).
o-Phenylenediamine Free Base is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3 N HCl or 3 M H2SO4 and read at 492 nm.
Biochem/physiol Actions
o-Phenylenediamine (o-PD) is a potent substrate for spectrophotometric horseradish peroxidase (HRP)-mediated enzyme-linked immunosorbent assay (ELISA), which gives 2,3-diaminophenazine (DAP) as a product of o-PD oxidation.
Preparation Note
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
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Danger
Hazard Classifications
Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
276.8 °F - closed cup
flash_point_c
136 °C - closed cup
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