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About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Product Name
PGO Enzyme Preparation, 1 G capsules
form
powder
solubility
H2O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)
storage temp.
2-8°C
Quality Level
Application
PGO Enzymes preparation has been used:
- in diet sampling and chemical analyses
- to determine plasma and follicular fluid glucose levels
- to measure glucose content in fractions of retrograded debranched rice starch
Tested and found suitable for use in the quantitation of glucose.
Biochem/physiol Actions
PGO Enzymes are used for the quantitative, enzymatic determination of glucose in aqueous solutions such as serum. The reactions are normally monitored at 425-475 nm utilizing o-dianisidine as a colorimetric substrate. Glucose oxidase methods eliminate the effects of interfering substances. Contents of one capsule includes 100 units peroxidase and 500 units glucose oxidase and buffer salts.
General description
Each capsule contains 500 units of glucose oxidase (Aspergilus niger), 100 purpurogallin units of peroxidase (horseradish), and buffer salts. The reaction produces oxidized o-Dianisidine, which is brown. The intensity of the brown color measured at 425-475 nm is proportional to the original glucose concentration.
Preparation Note
The PGO enzymes solution is prepared by adding the contents of 1 capsule of PGO enzymes to 100 mL of water in an amber bottle. Bottle should be inverted several times with gentle shaking to dissolve. PGO Enzymes solution should be stored at 2-8 °C. The solution is stable for up to 1 month unless turbidity develops, and for at least 6 months at –20 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Effect of dehydration methods on digested starch fractions of retrograded debranched rice starch
Boonna S, et al.
Journal of Science and Technology, 17(4), 359-368 (2010)
Effects of reducing dietary starch content by replacing barley grain with wheat dried distillers grains plus solubles in dairy cow rations on ovarian function
Subramaniam E, et al.
Journal of Dairy Science, 99, 2762-2774 (2016)
W Z Yang et al.
Journal of animal science, 90(4), 1301-1310 (2011-11-22)
A study was conducted to evaluate feed intake, ADG, carcass quality, eating behavior, and blood metabolites in feedlot beef steers fed diets that varied in proportion of wheat dried distillers grains with solubles (DDGS) replacing barley grain or barley silage.
Reza Salehi et al.
Reproductive biology and endocrinology : RB&E, 13, 69-69 (2015-07-04)
The objective was to determine the effect of prepartum diets supplemented with rolled canola seed (high in oleic acid) or sunflower seed (high in linoleic acid) on luteinizing hormone (LH) pulsatility and gonadotropin releasing hormone (GnRH)-induced LH release during early
M T Harper et al.
Journal of dairy science, 100(8), 6151-6163 (2017-06-12)
The objective of this experiment was to partially replace corn silage with 2 alternative forages, wheat (Triticum aestivum) or triticale (X Triticosecale) silages at 10% of the diet dry matter (DM), and investigate the effects on dairy cow productivity, nutrient
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