S9396

Sphingomyelinase from Bacillus cereus

Name: Sphingomyelinase from <I>Bacillus cereus</I>
Brand: SIGMA

buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

Synonym(s):

Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase

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About This Item

CAS Number:

9031-54-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.1.4.12 (BRENDA, IUBMB)

MDL number:

MFCD00132362

Product Name

Sphingomyelinase from Bacillus cereus, buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

Quality Level

200

form

buffered aqueous glycerol solution

specific activity

≥100 units/mg protein (Lowry)

mol wt

34.2 kDa

foreign activity

Phospholipase C ≤1%

storage temp.

2-8°C

Gene Information

Bacillus cereus ATCC 10987 ... spH(2751530)

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Application

Sphingomyelinase from Bacillus cereus has been used:

in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines
in lipoprotein modification for the generation of cholesterol crystals in vitro
to aggregate LDL (low-density lipoprotein)

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems.

Biochem/physiol Actions

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin. SMPD has the ability to prevent colorectal carcinoma and colitis.

General description

Sphingomyelinase (SMase) is a hydrolase, of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.

Other Notes

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

Physical form

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000464513

0000464512

0000464511

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Use of X-ray scattering to aid the design and delivery of membrane-active drugs.

G Pabst et al.

European biophysics journal : EBJ, 41(10), 915-929 (2012-06-05)

Biological membranes can be targets for compounds that either disrupt their barrier function or affect protein function via membrane-mediated processes. Biophysical studies on membrane-mimetic systems composed of membrane lipids have contributed substantially to our knowledge on the pertaining membrane physics

Analysis of the Effect of Sphingomyelinase on Rubella Virus Infectivity in Two Cell Lines

Otsuki N, et al.

Journal of virology (2017)

Extracellular lipids accumulate in human carotid arteries as distinct three-dimensional structures and have proinflammatory properties

Lehti S, et al.

The American Journal of Pathology, 188(2), 525-538 (2018)

Antioxidants inhibit low density lipoprotein oxidation less at lysosomal pH: A possible explanation as to why the clinical trials of antioxidants might have failed

Ahmad F and Leake D S

Chemistry and Physics of Lipids, 213, 13-24 (2018)

The Bacillus cereus group

Molecular Medical Microbiology, 1041-1078 (2015)

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