487655
p-Nitrophenyl Phosphate, DiTris Salt
Excellent substrate for alkaline phosphatase-based ELISA assays.
Synonym(s):
p-Nitrophenyl Phosphate, DiTris Salt, pNPP, 2Tris
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About This Item
Empirical Formula (Hill Notation):
C14H28N3O12P
CAS Number:
Molecular Weight:
461.36
UNSPSC Code:
41116158
NACRES:
NA.77
MDL number:
Assay:
≥98% (HPLC)
Form:
solid
Storage condition:
OK to freeze
Quality Segment
assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤0.05% Free-p-Nitrophenol
color
off-white to pale yellow
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
SMILES string
[P](=O)([O-])([O-])Oc1ccc(cc1)[N+](=O)[O-].[N+H3]C(CO)(CO)CO.[N+H3]C(CO)(CO)CO
InChI
1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
InChI key
XXAXKCWOTRABOW-UHFFFAOYSA-N
General description
Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and end point determinations can be performed.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Substrate for alkaline phosphatase-based ELISA assays
Substrate for alkaline phosphatase-based ELISA assays
Product does not compete with ATP.
Reversible: no
Preparation Note
Unstable in solution; reconstitute just prior to use.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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