MAB3400
Anti-Vimentin Antibody
CHEMICON®, mouse monoclonal, V9
Synonym(s):
Vimentin antibody
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
V9, monoclonal
Species reactivity:
monkey, feline, pig, bovine, rat, human, chicken, canine
Application:
ICC, IF, IHC (p), WB
Technique(s):
immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
Citations:
90
Uniprot accession no.:
Product Name
Anti-Vimentin Antibody, clone V9, clone V9, Chemicon®, from mouse
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
V9, monoclonal
species reactivity
monkey, feline, pig, bovine, rat, human, chicken, canine
should not react with
mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
Quality Level
Gene Information
human ... VIM(7431)
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Analysis Note
Evaluated by Western Blot on HeLa lysates.
Western Blot:
1:1000 dilution of this antibody detected VIMENTIN on 10 μg of HeLa lysates
Western Blot:
1:1000 dilution of this antibody detected VIMENTIN on 10 μg of HeLa lysates
Application
Anti-Vimentin Antibody, clone V9 is an antibody against Vimentin for use in IC, IH, IH(P) & WB.
Immunoblotting: A dilution of 1:1000 was used in a previous lot.
Immunohistochemistry: 5-10 μg/mL was used on a previous lot; can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antivbody reactivity.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Vimentin in human breast tissue and patient-derived triple negative breast cancer organoid sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Vimentin in human liver tissue, iPSC-derived liver organoid progenitor, and mature liver organoid sections.
Immunofluorescence Analysis: A 1:200 dilution from a representative lot detected Vimentin in human small intestine and differentiated duodenum intestinal organoid tissue sections.
Immunocytochemistry: 5-10 μg/mL of a previous lot was used in IC.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-10 μg/mL was used on a previous lot; can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antivbody reactivity.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Vimentin in human breast tissue and patient-derived triple negative breast cancer organoid sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Vimentin in human liver tissue, iPSC-derived liver organoid progenitor, and mature liver organoid sections.
Immunofluorescence Analysis: A 1:200 dilution from a representative lot detected Vimentin in human small intestine and differentiated duodenum intestinal organoid tissue sections.
Immunocytochemistry: 5-10 μg/mL of a previous lot was used in IC.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes a 57-60 kDa protein. Shows no cross reactivity with other closely related intermediate filament proteins including desmin and GFAP. In tissue sections, this reagent shows positive reaction on connective tissue, endothelial cells, vascular smooth muscle cells and astrocytes. In tissue culture, typical intermediate filament profiles are seen in fibroblasts and other cell types, which contain vimentin
General description
57-60 kDa
Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin.
Immunogen
Purified vimentin from pig eye lens
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB3410
Physical form
Format: Purified
Purified mouse monoclonal IgG1 presented in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Noelia López-Montero et al.
Autophagy, 12(10), 1886-1901 (2016-08-04)
Xenophagy has been studied in epithelial cells infected with Salmonella enterica serovar Typhimurium (S. Typhimurium). Distinct autophagy receptors target this pathogen to degradation after interacting with ubiquitin on the surface of cytosolic bacteria, and the phagophore- and autophagosome-associated protein MAP1LC3/LC3.
E Mato et al.
Journal of molecular endocrinology, 52(3), 289-300 (2014-03-20)
Tumor malignancy is associated with the epithelial-mesenchymal transition (EMT) process and resistance to chemotherapy. However, little is known about the relationship between the EMT and the multidrug-resistance gene in thyroid tumor progression. We investigated whether the expression of the ABCG2/BCRP
Muhammad Umar Cheema et al.
PloS one, 9(7), e101258-e101258 (2014-07-08)
Prolonged elevations of plasma aldosterone levels are associated with renal pathogenesis. We hypothesized that renal distress could be imposed by an augmented aldosterone-induced protein turnover challenging cellular protein degradation systems of the renal tubular cells. Cellular accumulation of specific protein
Dolores D Mruk et al.
Biology of reproduction, 80(3), 590-601 (2008-12-17)
During spermatogenesis, leptotene spermatocytes residing in the basal compartment of the seminiferous epithelium must traverse the blood-testis barrier (BTB) to gain entry into the adluminal compartment for further development. At the same time, these as well as other germ cell
Shih-Pei Lin et al.
PloS one, 7(11), e49605-e49605 (2012-11-28)
Hypoxia and serum depletion are common features of solid tumors that occur upon antiangiogenesis, irradiation and chemotherapy across a wide variety of malignancies. Here we show that tumor cells expressing CD133, a marker for colorectal cancer initiating or stem cells
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB3400 | 04053252726712 |
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