I9274
Invertase from baker′s yeast (S. cerevisiae)
200-300 units/mg solid
Synonym(s):
β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase
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About This Item
CAS Number:
UNSPSC Code:
12164500
NACRES:
NA.21
EC Number:
232-615-7
MDL number:
Specific activity:
200-300 units/mg solid
Quality Level
specific activity
200-300 units/mg solid
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
foreign activity
α-glucosidase (maltase), phosphoglucomutase, phosphoglucose isomerase ≤0.01%, 6-phosphogluconic dehydrogenase, α-galactosidase, β-galactosidase, and NADH oxidase ≤0.001%
storage temp.
2-8°C
General description
Invertase is encoded by the SUC2 (Saccharomyces cerevisiae S288C) gene. In yeast this enzyme exist in: glycosylated (secreted) and non-glycosylated (intracellular) form. The external invertase is composed of carbohydrate (50%) and 60,000 D subunits.
Application
Used in the production of confectionary foods and artificial honey.
Invertase from baker′s yeast (S. cerevisiae) has been used:
- in liquid media (YES) and is used to determine the invertase activity
- supplemented to the reaction mixture in the extraction and analysis of sugars and starch
- for sucrose hydrolysis
Biochem/physiol Actions
Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.
Other Notes
One unit will hydrolyze 1.0 μmole sucrose to glucose and fructose per minute at pH 4.6 at 25 °C.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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