Skip to Content
Merck

P3504

Ponceau S

Powder

Synonym(s):

3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):
C22H12N4Na4O13S4
CAS Number:
Molecular Weight:
760.57
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
228-319-2
MDL number:
Colour Index Number:
27195
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


Product Name

Ponceau S, BioReagent, suitable for electrophoresis

product line

BioReagent

Quality Level

form

powder

technique(s)

electrophoresis: suitable

color

red to very dark red

solubility

H2O: 1 mg/mL

ε (extinction coefficient)

≥330000 at 518-522 nm in deionized water at 0.015 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

InChI key

KQHKSGRIBYJYFX-WNDMICSESA-J

General description

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection.
The following are two common stain formulations:
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid

Application

Ponceau S has been used for membrane staining (Western blotting.


Still not finding the right product?

Explore all of our products under Ponceau S


Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library



Sex difference in the expression of DNA methyltransferase 3a (DNMT3a) in the rat amygdala during development
MH Kolodkin and AP Auger
Journal of Neuroendocrinology (2011)
Characterization of stress response in human retinal epithelial cells
Journal of Cellular and Molecular Medicine (2012)
Bernard L. Horecker, Earl R. Stadtman
Current Topics in Cellular Regulation (2014)