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About This Item
Empirical Formula (Hill Notation):
C14H14O4
CAS Number:
Molecular Weight:
246.26
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
241-694-7
MDL number:
Beilstein/REAXYS Number:
227973
Product Name
4-Methylumbelliferyl butyrate, suitable for fluorescence, ≥95% (HPCE)
Quality Level
assay
≥95% (HPCE)
form
powder or crystals
mp
90-92 °C (lit.)
solubility
DMF: soluble, DMSO: soluble
fluorescence
λex 312 nm in methanol, λex 360 nm; λem 449 nm (Reaction product), λex 365 nm; λem 445 nm in 0.1 M Tris pH 8.0 (Lipase)
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
CCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1
InChI
1S/C14H14O4/c1-3-4-13(15)17-10-5-6-11-9(2)7-14(16)18-12(11)8-10/h5-8H,3-4H2,1-2H3
InChI key
WKPUJZVCZXWKCK-UHFFFAOYSA-N
Application
suitable as fluorogenic substrate for esterases/lipases
Other Notes
Fluorogenic substrate for butyrate esterase
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Determination of lipase activity using image analysis.
Marcelo Müller-Santos et al.
Analytical biochemistry, 351(2), 305-307 (2006-03-10)
M Vaneechoutte et al.
Journal of clinical microbiology, 26(6), 1227-1228 (1988-06-01)
Branhamella catarrhalis can be distinguished from Neisseria spp. by the presence of butyrate esterase. This enzyme can be rapidly detected when 4-methylumbelliferyl butyrate is used as the substrate. All B. catarrhalis strains tested gave a positive fluorescence reaction within 5
Neda Gilannejad et al.
Animals : an open access journal from MDPI, 10(5) (2020-05-24)
Thick-lipped grey mullet (Chelon labrosus) is a candidate for sustainable aquaculture due to its omnivorous/detritivorous feeding habit. This work aimed to evaluate its digestive and growth potentials from larval to early juvenile stages. To attain these objectives the activity of