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About This Item
Empirical Formula (Hill Notation):
C24H34ClNO18
CAS Number:
Molecular Weight:
659.98
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32
MDL number:
Product Name
2-Chloro-4-nitrophenyl-α-D-maltotrioside, ≥95% (HPLC)
Quality Level
assay
≥95% (HPLC)
form
powder or crystals
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O[C@@H]2CO)O[C@H]3[C@H](O)[C@@H](O)[C@H](O[C@@H]3CO)Oc4ccc(cc4Cl)[N+]([O-])=O)[C@H](O)[C@@H](O)[C@@H]1O
InChI
1S/C24H34ClNO18/c25-8-3-7(26(37)38)1-2-9(8)39-22-18(35)15(32)20(11(5-28)41-22)44-24-19(36)16(33)21(12(6-29)42-24)43-23-17(34)14(31)13(30)10(4-27)40-23/h1-3,10-24,27-36H,4-6H2/t10-,11-,12-,13-,14+,15-,16-,17-,18-,19-,20-,21-,22+,23-,24-/m1/s1
InChI key
KMYYNUOXSFGLNX-XFNLHOCBSA-N
Application
2-Chloro-4-nitrophenyl-α-D-maltotrioside has been used as a substrate in assays to determine the enzymatic activity of α-amylase.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Kinetic determination of amylase on microtiter plates: an improved substrate.
J Laine et al.
Pancreas, 13(2), 217-217 (1996-08-01)
alpha-Amylase and alpha-glucosidase inhibitory saponins from Polyscias fruticosa leaves
Hanh TTH, et al.
Journal of Chemistry (2016)
E S Winn-Deen et al.
Clinical chemistry, 34(10), 2005-2008 (1988-10-01)
We describe a direct colorimetric assay for alpha-amylase, with 2-chloro-4-nitrophenyl-alpha-maltotrioside as substrate. Both human pancreatic and salivary amylase split this substrate without the use of helper enzymes, yielding free 2-chloro-4-nitrophenol, which is monitored at 405 nm. The performance of this