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Merck

B4875

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride

trypsin substrate, chromogenic, elastase substrate, ≥98%, powder

Synonym(s):

Nα-Benzoyl-DL-arginine p-nitroanilide hydrochloride, BANI, BAPNA

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About This Item

Empirical Formula (Hill Notation):
C19H22N6O4 · HCl
CAS Number:
911-77-3
Molecular Weight:
434.88
NACRES:
NA.32
PubChem Substance ID:
24891801
UNSPSC Code:
12352204
EC Number:
213-011-2
MDL number:
MFCD00012846
Beilstein/REAXYS Number:
4081878

Key Documents

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Product Name

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, ≥98%

Quality Level

200

assay

≥98%

form

powder

color

, white to light yellow to light brown

solubility

DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)

storage temp.

−20°C

SMILES string

Cl[H].NC(=N)NCCCC(NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O

InChI

1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H

InChI key

DEOKFPFLXFNAON-UHFFFAOYSA-N

General description

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride (DL-BAPNA) is a chromogenic substrate for proteolytic enzymes such as trypsin, amidase, and balterobin. Hydrolysis of DL-BAPNA at the bond between the arginine and the p-nitroaniline moieties releases the chromophore p-nitroaniline, which can be detected by colorimetric analysis.
A chromogenic trypsin substrate.

Application

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride has been used as a substrate for trypsin and elastase in enzyme analysis.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP3670
BCCN4338
BCCN1610
BCCN0290
BCCL6610

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I Kurtovic et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 143(4), 432-440 (2006-02-07)
A trypsin fraction was isolated from the pyloric ceca of New Zealand farmed chinook salmon (Oncorhynchus tshawytscha) by ammonium sulfate fractionation, acetone precipitation and affinity chromatography. The chinook salmon enzyme hydrolyzed the trypsin-specific synthetic substrate benzoyl-DL-arginine-p-nitroanilide (DL-BAPNA), and was inhibited
Anju Singh et al.
Journal of biomolecular structure & dynamics, 1-14 (2017-09-21)
Regulatory regions in human genome, enriched in guanine-rich DNA sequences have the propensity to fold into G-quadruplex structures. On exploring the genome for search of G-tracts, it was interesting to find that promoter of Human Myosin Gene (MYH7) contains a
Guilherme M Zanatta et al.
Journal of periodontology, 77(3), 498-505 (2006-03-04)
The aim of the present study was to evaluate the clinical effects of one-stage periodontal debridement with an ultrasonic instrument, associated with 0.5% povidone (pvp)-iodine irrigation in patients with chronic periodontitis. Forty-five patients were randomly assigned into three groups: the
Nguyen Van Quan et al.
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 27(5), 643-649 (2019-07-13)
This study was the first to detect the presence of the two compounds momilactone A (MA) and momilactone B (MB) in rice bran using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS). By in vitro assays, both MA and MB exhibited potent inhibitory
Carola Engler et al.
PloS one, 4(5), e5553-e5553 (2009-05-14)
We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct. This protocol is based on the
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