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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Technique(s):
ELISA: suitable
Species reactivity:
-
Application:
ELISA
Citations:
25
General description
Non-radioactive, competitive immunoassay for the quantitation of cAMP in biological fluids (serum, plasma, and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cAMP to competitively bind cAMP or cAMP which has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cAMP present in the well.
Application
cAMP Enzyme Immunoassay Kit has been used to quantify the cAMP level.
Other Notes
Please refer to the attached Protocolfor details.
Kit Components Only
Product No.
Description
- Acetic Anhydride 1 x 1
- Assay Buffer 2 1 x 30
- cAMP-Alkaline Phosphatase Conjugate 1 x 5
- cAMP Assay Layout Sheet 1 x 1
- cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5
- 5 Cycle Logit-Log Paper 1 x 1
- Cyclic AMP Standard 1 x 0.5
See All (13)
signalword
Danger
target_organs
Respiratory system
Storage Class
3 - Flammable liquids
wgk
WGK 3
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1A - STOT SE 3
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