D0550
3,5-Dinitrosalicylic acid
used in colorimetric determination of reducing sugars
Synonym(s):
3,5-Dinitro-2-hydroxybenzoic acid, DNS
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About This Item
Linear Formula:
(O2N)2C6H2-2-(OH)CO2H
CAS Number:
Molecular Weight:
228.12
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
210-204-3
Beilstein/REAXYS Number:
2220661
MDL number:
Assay:
≥98% (HPLC and titration)
Form:
powder
Quality Level
assay
≥98% (HPLC and titration)
form
powder
mp
168-172 °C (lit.)
solubility
H2O: 50 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
OC(=O)c1cc(cc(c1O)[N+]([O-])=O)[N+]([O-])=O
InChI
1S/C7H4N2O7/c10-6-4(7(11)12)1-3(8(13)14)2-5(6)9(15)16/h1-2,10H,(H,11,12)
InChI key
LWFUFLREGJMOIZ-UHFFFAOYSA-N
Application
3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars.
Biochem/physiol Actions
3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method.
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1
Storage Class
4.1A - Other explosive hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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Studies on the ecology of actinomycetes in an agricultural soil amended with organic residues: II. Assessment of enzymatic activities of Actinomycetales isolates.
Mokni-Tlili S, et al.
World Journal of Microbiology & Biotechnology, 27, 2251-2259 (2011)
Comparison of two methods for assaying reducing sugars in the determination of carbohydrase activities.
Gusakov, A.V., et al.
International Journal of Antimicrobial Agents, e283658-e283658 (2011)
Use of dinitrosalicylic acid reagent for determination of reducing sugar.
Miller, G.L.
Analytical Chemistry, 31, 426-428 (1959)