D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

Name: Deoxyribonuclease I RNase-free solution from bovine pancreas
Brand: SIGMA

Synonym(s):

DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I

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About This Item

CAS Number:

9003-98-9

UNSPSC Code:

12352204

NACRES:

NA.53

EC Number:

232-667-0

MDL number:

MFCD00130918

EC Number:

3.1.21.1 (BRENDA, IUBMB)

biological source

bovine pancreas

grade

Molecular Biology

form

buffered aqueous glycerol solution

specific activity

10000 units/mg protein

mol wt

29.1 kDa

concentration

5—15 mg protein/mL

technique(s)

DNA extraction: suitable

suitability

suitable for molecular biology

UniProt accession no.

P00639

application(s)

cell analysis

foreign activity

RNase, none detected

storage temp.

−20°C

Quality Level

200

Gene Information

cow ... DNASE1(282217)

Application

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

General description

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg²⁺, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn²⁺, both strands can be cleaved.

Other Notes

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

One unit will cause a ΔA₂₆₀ of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000454444

SLCQ3894

0000343115

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Stage-Specific Expression Profiles of Anti-Mullerian Hormone and its Type II Receptor in Germ Cells during Spermatogenic Cycle of Rats

Ohyama K, et al.

Journal of Steroids & Hormonal Science, 8(187), 2-2 (2017)

Hypercholesterolemia Impairs Clearance of Neutrophil Extracellular Traps and Promotes Inflammation and Atherosclerotic Plaque Progression.

Umesh Kumar Dhawan et al.

Arteriosclerosis, thrombosis, and vascular biology, 41(10), 2598-2615 (2021-08-06)

Objective: Hypercholesterolemia-induced NETosis and accumulation of neutrophil extracellular traps (NETs) in the atherosclerotic lesion exacerbates inflammation and is causally implicated in plaque progression. We investigated whether hypercholesterolemia additionally impairs the clearance of NETs mediated by endonucleases such as DNase1 and

A Deoxyribonuclease from Chlamydomonas reinhardii:Purification and Properties

TAIT GCL and HARRIS WJ

European Journal of Biochemistry, 75(2), 357-364 (1977)

A fast signal-induced activation of Poly(ADP-ribose) polymerase: a novel downstream target of phospholipase c.

S Homburg et al.

The Journal of cell biology, 150(2), 293-307 (2000-07-26)

We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARP, an abundant, highly conserved, chromatin-bound protein

Expression of anti-Mullerian hormone and its type II receptor in germ cells of maturing rat testis