G3907
Glutathione−Agarose
set of 3 pre-packed columns (2.5 ml each), (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)
Synonym(s):
GSH-agarose, S-linked glutathione agarose
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About This Item
Matrix:
cross-linked 4% beaded agarose
Quality Level
form
(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)
analyte chemical class(es)
proteins (GST)
packaging
set of 3 pre-packed columns (2.5 ml each)
technique(s)
immunoprecipitation (IP): suitable, protein purification: suitable
matrix
cross-linked 4% beaded agarose
storage temp.
2-8°C
General description
The resin in these columns consists of glutathione attached through the sulfur to epoxy activated, 4% cross-linked beaded agarose resulting in a 12 atom spacer.
Application
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of glutathione binding enzymes such as glutathione-S-transferase, glutathione peroxidase, and glyoxalase I.
The product was used in the design, synthesis and biological evaluation of new tryptamine and tetrahydro-β-carboline-based selective inhibitors of CDK4. It was also used to study Fascaplysin-inspired diindolyls as selective inhibitors of CDK4/cyclin D1.
The product was used in the design, synthesis and biological evaluation of new tryptamine and tetrahydro-β-carboline-based selective inhibitors of CDK4. It was also used to study Fascaplysin-inspired diindolyls as selective inhibitors of CDK4/cyclin D1.
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.
Physical form
1:1 suspension in a 0.5 M NaCl + 20% ethanol solutionl
Other Notes
Suspension of Product No. G 4510
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Storage Class
10 - Combustible liquids
flash_point_f
180.1 °F
flash_point_c
82.3 °C
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Related Content
Carine Aubry et al.
Organic & biomolecular chemistry, 4(5), 787-801 (2006-02-24)
We present the design, synthesis, and biological activity of three classes of tryptamine derivatives, which are non-planar analogues of the toxic anti-cancer agent fascaplysin. We show these compounds to be selective inhibitors of CDK4 over CDK2, the most active compound
F Toribio et al.
Journal of chromatography. B, Biomedical applications, 684(1-2), 77-97 (1996-09-20)
The different preparative techniques and related analytical methods used for purification of glutathione peroxidase, glutathione transferase and glutathione reductase, described in papers published in the last ten years, have been reviewed in this article. Among the different purification techniques, chromatography
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