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About This Item
CAS Number:
NACRES:
NA.56
PubChem Substance ID:
UNSPSC Code:
47101511
MDL number:
form
aqueous ethanol suspension
Quality Level
technique(s)
activity assay: suitable
particle size
40-160 μm (wet)
pore size
~1,000,000 Da exclusion limit
pH
2—12
capacity
100-140 μeq/mL, gel
storage temp.
2-8°C
SMILES string
OC[C@@H]1O[C@@H](O[C@H]2[C@@H](O)[C@H](O)[C@@H](O)O[C@H]2CO)[C@@H](O)[C@H](O)[C@H]1O
InChI
1S/C12H22O11/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17/h3-20H,1-2H2/t3?,4?,5?,6?,7?,8?,9?,10-,11?,12+/m1/s1
InChI key
GUBGYTABKSRVRQ-WFVLMXAXSA-N
General description
I6505-500ML′s update product number is GE17-0500-01
Application
Diethylaminoethyl (DEAE) Sephacel® is used in protein chromatography, ion exchange chromatography, and anion exchange media. It has been used to study sperm motility, sperm capacitation and sperm-oocyte interactions. DEAE Sephacel has also been used to study patients with immunoglobulin A (IgA) nephropathy. Diethylaminoethyl–Sephacel® has been used:
- in the purification of media samples by ion-exchange chromatography
- in the isolation of glycosaminoglycans (GAG) from cultured animal cells
- in the purification of cytotoxic fractions from Tritrichomonas foetus isolates
Physical form
Suspension in 20% ethanol
Legal Information
Sephacel is a registered trademark of Cytiva
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
129.2 °F
flash_point_c
54 °C
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C M Rembold et al.
Biochimica et biophysica acta, 1500(3), 257-264 (2000-03-04)
Nitrovasodilators, high extracellular Mg(2+), and some other relaxing agents can cause smooth muscle relaxation without reductions in myosin regulatory light chain (MRLC) phosphorylation. Relaxations without MRLC dephosphorylation suggest that other regulatory systems, beyond MRLC phosphorylation, are present in smooth muscle.
Yuan-Hong Wang et al.
Acta biochimica et biophysica Sinica, 38(9), 633-638 (2006-09-06)
Extracellular alginate lyase secreted by marine Vibrio sp. YWA, isolated from decayed Laminaria japonica, was purified by a combination of ammonium sulfate precipitation and diethylaminoethyl-Sephacel column chromatography. The results show that the molecular mass of alginate lyase was approximately 62.5
A Ortiz et al.
Nucleic acids research, 24(16), 3280-3281 (1996-08-15)
A simple method is presented to circumvent laborious and time consuming electrophoretic separations of specific PCR amplification products. Specific target DNA is amplified using nucleotides labelled with DIG-dUTP or biotin-dCTP. The labelled PCR products are separated from unincorporated nucleotides or
