L3892
Lectin from Triticum vulgaris (wheat)
peroxidase conjugate, lyophilized powder
Synonym(s):
WGA, Wheat germ agglutinin
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Product Name
Lectin from Triticum vulgaris (wheat), peroxidase conjugate, lyophilized powder
biological source
Triticum vulgaris
conjugate
peroxidase conjugate
assay
≥85% protein basis (Warburg-Christian)
form
lyophilized powder
potency
<40 μg/mL agglutination activity
peroxidase activity
50-200 units/mg protein
composition
Protein, ~90% modified Warburg-Christian
storage temp.
−20°C
Quality Level
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Application
Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Other Notes
One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Packaging
Package size based on protein content.
Physical form
Contains citrate buffer
Preparation Note
Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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